Finger-Prick Whole Blood Cryptococcal Antigen Lateral Flow Assay for the Diagnosis of Cryptococcosis in HIV-Negative Patients: A Case Series Study in Two Tertiary Centers in São Paulo, Brazil
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Citações na Scopus
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Tipo de produção
article
Data de publicação
2023
Título da Revista
ISSN da Revista
Título do Volume
Editora
MDPI
Autores
OLIVEIRA, Fernanda Gurgel
VIEIRA, Marcela
PEREIRA, Luisa
LUCAS JUNIOR, Rodovaldo M.
BOULWARE, David R.
Citação
JOURNAL OF FUNGI, v.9, n.12, article ID 1140, 15p, 2023
Resumo
Cryptococcosis in HIV-negative patients can be an opportunistic or endemic disease. There are no published studies on the use of the finger-prick whole blood (point-of-care) cryptococcal antigen lateral flow assay (CrAg LFA) for diagnosing cryptococcosis in HIV-negative patients. We conducted a case series study of HIV-negative patients with cryptococcosis in two centers in S & atilde;o Paulo, Brazil. The objectives were to identify the sensitivity of a finger-prick whole blood CrAg LFA and to describe the main characteristics of this population. We identified 30 HIV-negative patients with cryptococcosis [19 (63%), male; median age, 47 years]. Ten (33%) patients were immunosuppressed, ten (33%) had other comorbidities, and ten (33%) were apparently immunocompetent and without comorbidities. The distribution of the sites of cryptococcosis was as follows: the central nervous system, 90% (n = 27); pulmonary, 43% (n = 13); and other extrapulmonary sites, 40% (n = 12). The sensitivity of the finger-prick whole blood CrAg LFA for the diagnosis of cryptococcosis was 97% (29/30). Among 26 participants with cryptococcal meningitis, the sensitivity of testing cerebrospinal fluid was as follows: CrAg latex agglutination, 77% (20/26); CrAg LFA, 96% (25/26); and culture, 81% (21/26). Culture speciation identified Cryptococcus gattii in 16 (62%) cases, and all had a positive finger-prick whole blood CrAg LFA. This test presented high sensitivity to the diagnosis of cryptococcosis in HIV-negative patients, including those caused by C. gattii.
Palavras-chave
cryptococcosis, cryptococcal meningitis, diagnosis, point-of-care testing, lateral flow assay, Cryptococcus gattii, Brazil
Referências
- [Anonymous], CrAg® LFA Cryptococcal Antigen
- Beardsley J, 2019, J FUNGI, V5, DOI 10.3390/jof5030071
- Boyd K, 2022, MICROBIOL SPECTR, V10, DOI 10.1128/spectrum.01075-22
- Bridge S, 2021, J MYCOL MED, V31, DOI 10.1016/j.mycmed.2021.101170
- Chen SCA, 2014, CLIN MICROBIOL REV, V27, P980, DOI 10.1128/CMR.00126-13
- Coussement Julien, 2023, Clin Infect Dis, V77, P976, DOI 10.1093/cid/ciad321
- DeBess E., 2010, Morbidity and Mortality Weekly Report, V59, P865
- Donnelly JP, 2020, CLIN INFECT DIS, V71, P1367, DOI 10.1093/cid/ciz1008
- Drain PK, 2014, LANCET INFECT DIS, V14, P239, DOI 10.1016/S1473-3099(13)70250-0
- Dubbels M, 2017, J CLIN MICROBIOL, V55, P2472, DOI 10.1128/JCM.00751-17
- Economic Commission for Latin America and the Caribbean (ECLAC), 2005, The Millennium Development Goals: A Latin America and Caribbean Perspective
- Firacative C, 2018, MEM I OSWALDO CRUZ, V113, DOI 10.1590/0074-02760170554
- Gushiken AC, 2021, INFECT DIS CLIN N AM, V35, P493, DOI 10.1016/j.idc.2021.03.012
- Hevey MA, 2020, J CLIN MICROBIOL, V58, DOI 10.1128/JCM.01563-20
- Ibe C, 2023, MED MYCOL, V61, DOI 10.1093/mmy/myad049
- Jarvis JN, 2008, SEMIN RESP CRIT CARE, V29, P141, DOI 10.1055/s-2008-1063853
- Jitmuang A, 2016, J CLIN MICROBIOL, V54, P460, DOI 10.1128/JCM.02223-15
- Kiertiburanakul S, 2006, INT J INFECT DIS, V10, P72, DOI 10.1016/j.ijid.2004.12.004
- Kozel Thomas R, 2012, Expert Opin Med Diagn, V6, P245, DOI 10.1517/17530059.2012.681300
- Kwon-Chung KJ, 2017, MSPHERE, V2, DOI 10.1128/mSphere.00357-16
- Macrae C, 2023, BMC INFECT DIS, V23, DOI 10.1186/s12879-023-08135-w
- Maziarz EK, 2016, INFECT DIS CLIN N AM, V30, P179, DOI 10.1016/j.idc.2015.10.006
- Min JQ, 2020, BMC INFECT DIS, V20, DOI 10.1186/s12879-020-4818-1
- Pappas Peter G, 2013, Trans Am Clin Climatol Assoc, V124, P61
- Assy JGPL, 2021, MED MYCOL, V59, P909, DOI 10.1093/mmy/myab021
- Prado M, 2009, MEM I OSWALDO CRUZ, V104, P513, DOI 10.1590/S0074-02762009000300019
- Silva GD, 2022, ARQ NEURO-PSIQUIAT, V80, P1167, DOI 10.1055/s-0042-1758645
- Soares EA, 2019, PLOS NEGLECT TROP D, V13, DOI 10.1371/journal.pntd.0007569
- Trilles L, 2008, MEM I OSWALDO CRUZ, V103, P455, DOI 10.1590/S0074-02762008000500008
- Vidal JE, 2013, BRAZ J INFECT DIS, V17, P353, DOI 10.1016/j.bjid.2012.10.020
- Wake RM, 2018, JAIDS-J ACQ IMM DEF, V78, P574, DOI [10.1097/QAI.0000000000001721, 10.1097/qai.0000000000001721]
- WHO, 2022, Fungal priority pathogen list to guide research, development and public health action
- Williams DA, 2015, CLIN INFECT DIS, V61, P464, DOI 10.1093/cid/civ263
- World Health Organization, 2022, GUIDELINES DIAGNOSIN