MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorNAVARRO, Isabela Cunha
dc.contributor.authorFERREIRA, Frederico Moraes
dc.contributor.authorNAKAYA, Helder I.
dc.contributor.authorBARON, Monique Andrade
dc.contributor.authorVILAR-PEREIRA, Glaucia
dc.contributor.authorPEREIRA, Isabela Resende
dc.contributor.authorSILVA, Ana Maria Goncalves
dc.contributor.authorREAL, Juliana Monte
dc.contributor.authorBRITO, Thales De
dc.contributor.authorCHEVILLARD, Christophe
dc.contributor.authorLANNES-VIEIRA, Joseli
dc.contributor.authorKALIL, Jorge
dc.contributor.authorCUNHA-NETO, Edecio
dc.contributor.authorFERREIRA, Ludmila Rodrigues Pinto
dc.date.accessioned2015-10-26T16:32:29Z
dc.date.available2015-10-26T16:32:29Z
dc.date.issued2015
dc.description.abstractChagas disease is caused by the parasite Trypanosoma cruzi, and it begins with a short acute phase characterized by high parasitemia followed by a life-long chronic phase with scarce parasitism. Cardiac involvement is the most prominent manifestation, as 30% of infected subjects will develop abnormal ventricular repolarization with myocarditis, fibrosis and cardiomyocyte hypertrophy by undefined mechanisms. Nevertheless, follow-up studies in chagasic patients, as well as studies with murine models, suggest that the intensity of clinical symptoms and pathophysiological events that occur during the acute phase of disease are associated with the severity of cardiac disease observed during the chronic phase. In the present study we investigated the role of microRNAs (miRNAs) in the disease progression in response to T. cruzi infection, as alterations in miRNA levels are known to be associated with many cardiovascular disorders. We screened 641 rodent miRNAs in heart samples of mice during an acute infection with the Colombiana T.cruzi strain and identified multiple miRNAs significantly altered upon infection. Seventeen miRNAs were found significantly deregulated in all three analyzed time points post infection. Among these, six miRNAs had their expression correlated with clinical parameters relevant to the disease, such as parasitemia and maximal heart rate-corrected QT (QTc) interval. Computational analyses identified that the gene targets for these six miRNAs were involved in networks and signaling pathways related to increased ventricular depolarization and repolarization times, important factors for QTc interval prolongation. The data presented here will guide further studies about the contribution of microRNAs to Chagas heart disease pathogenesis.
dc.description.indexMEDLINE
dc.description.sponsorshipSao Paulo state Foundation for Scientific Research (FAPESP) [2012/08107-6, 2013/50302-3]
dc.description.sponsorshipBrazilian National Research Council (CNPq)
dc.description.sponsorshipNational Institute of Allergy and Infectious Disease [1P50AI098461-01]
dc.description.sponsorshipCNPq fellowship [151045/2013-5]
dc.description.sponsorshipFAPESP fellowship
dc.description.sponsorshipRio de Janeiro Foundation for Scientific Research (FAPERJ)
dc.description.sponsorshipMedical Investigation Laboratory
dc.description.sponsorshipCNPq [474234/20126-Universal, 302534/2008-3]
dc.description.sponsorshipFAPERJ [APQ1-E-26/111.756/2008, CNE/E-26/101.549/2010, E-26/110.153/2013, E-26/111.709/2013]
dc.description.sponsorshipNational Institute for Science and Technology for Vaccines (INCTV/CNPq) [403979/2012-9-DECIT]
dc.description.sponsorshipInstitut National de la Sante et de la Recherche Medicale (INSERM)
dc.identifier.citationPLOS NEGLECTED TROPICAL DISEASES, v.9, n.6, article ID e0003828, 17p, 2015
dc.identifier.doi10.1371/journal.pntd.0003828
dc.identifier.issn1935-2735
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/11817
dc.language.isoeng
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.ispartofPlos Neglected Tropical Diseases
dc.rightsopenAccess
dc.rights.holderCopyright PUBLIC LIBRARY SCIENCE
dc.subject.otherchronic chagas-disease
dc.subject.otheramerican trypanosomiasis
dc.subject.othergene-expression
dc.subject.otherparasite load
dc.subject.otherchronic phase
dc.subject.othercardiomyopathy
dc.subject.otherabnormalities
dc.subject.otherdysfunction
dc.subject.otherparameters
dc.subject.othermortality
dc.subject.wosInfectious Diseases
dc.subject.wosParasitology
dc.subject.wosTropical Medicine
dc.titleMicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice: Parasitological and Cardiological Outcomes
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.affiliation.countryFrança
hcfmusp.affiliation.countryisofr
hcfmusp.author.externalNAKAYA, Helder I.:Univ Sao Paulo, Dept Clin Anal & Toxicol, Sch Pharmaceut Sci, Sao Paulo, Brazil
hcfmusp.author.externalVILAR-PEREIRA, Glaucia:Fiocruz MS, Inst Oswaldo Cruz, Lab Biol Interact, BR-21045900 Rio De Janeiro, Brazil
hcfmusp.author.externalPEREIRA, Isabela Resende:Fiocruz MS, Inst Oswaldo Cruz, Lab Biol Interact, BR-21045900 Rio De Janeiro, Brazil
hcfmusp.author.externalREAL, Juliana Monte:Hosp Sirio Libanes, Sao Paulo, Brazil
hcfmusp.author.externalCHEVILLARD, Christophe:Aix Marseille Univ, Fac Med, INSERM, U906, Marseille, France
hcfmusp.author.externalLANNES-VIEIRA, Joseli:Fiocruz MS, Inst Oswaldo Cruz, Lab Biol Interact, BR-21045900 Rio De Janeiro, Brazil
hcfmusp.citation.scopus54
hcfmusp.contributor.author-fmusphcISABELA CUNHA NAVARRO
hcfmusp.contributor.author-fmusphcFREDERICO MORAES FERREIRA
hcfmusp.contributor.author-fmusphcMONIQUE ANDRADE BARON
hcfmusp.contributor.author-fmusphcANA MARIA GONCALVES DA SILVA
hcfmusp.contributor.author-fmusphcTHALES DE BRITO
hcfmusp.contributor.author-fmusphcJORGE ELIAS KALIL FILHO
hcfmusp.contributor.author-fmusphcEDECIO CUNHA NETO
hcfmusp.contributor.author-fmusphcLUDMILA RODRIGUES PINTO FERREIRA CAMARGO
hcfmusp.description.articlenumbere0003828
hcfmusp.description.issue6
hcfmusp.description.volume9
hcfmusp.origemWOS
hcfmusp.origem.pubmed26086673
hcfmusp.origem.scopus2-s2.0-84934779461
hcfmusp.origem.wosWOS:000357398100032
hcfmusp.publisher.citySAN FRANCISCO
hcfmusp.publisher.countryUSA
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