Micromorphology of the Dental Pulp Is Highly Preserved in Cancer Patients Who Underwent Head and Neck Radiotherapy
Carregando...
Citações na Scopus
31
Tipo de produção
article
Data de publicação
2014
Editora
ELSEVIER SCIENCE INC
Indexadores
Título da Revista
ISSN da Revista
Título do Volume
Métricas da Revista
Autores
FARIA, Karma Morais
BRANDAO, Thais Bianca
RIBEIRO, Ana Carolina Prado
VASCONCELLOS, Adriele Ferreira Gouvea
GROSS, Vanessa Cristina
ALMEIDA, Oslei Paes
LOPES, Marcio Ajudarte
Autor de Grupo de pesquisa
Editores
Coordenadores
Organizadores
Citação
JOURNAL OF ENDODONTICS, v.40, n.10, p.1553-1559, 2014
Resumo
Introduction: Teeth are often included in the radiation field during head and neck radiotherapy, and recent clinical evidence suggests that dental pulp is negatively affected by the direct effects of radiation, leading to impaired sensitivity of the dental pulp: Therefore, this study aimed to investigate the direct effects of radiation on the microvasculature, innervation, and extracellular matrix of the dental pulp of patients who have undergone head and neck radiotherapy. Methods: Twenty-three samples of dental pulp from patients who finished head and neck radiotherapy were analyzed. Samples were histologically processed and stained with hematoxylin-eosin for morphologic evaluation of the microvasculature, innervation, and extracellular matrix. Subsequently, immunohistochemical analysis of proteins related to vascularization (CD34 and smooth muscle actin), innervation (S-100, NCAM/CD56, and neurofilament), and extracellular matrix (vimentin) of the dental pulp was performed. Results: The morphologic study identified preservation of the microvasculature, nerve bundles, and components of the extracellular matrix in all studied samples. The immunohistochemical analysis confirmed the morphologic findings and showed a normal pattern of expression for the studied proteins in all samples. Conclusions: Direct effects of radiotherapy are not able to generate morphologic changes in the microvasculature, innervation, and extracellular matrix components of the dental pulp in head and neck cancer patients.
Palavras-chave
Cancer, dental pulp, radiation caries, radiotherapy, side effects
Referências
- Anneroth G, 1985, INT J ORAL SURG, V14, P169
- Barra MB, 2006, REV AMRIGS, V50, P173
- Bryson GJ, 2002, J CLIN PATHOL, V55, P535
- Carlos-Bregni R, 2009, PATHOL ONCOL RES, V15, P659, DOI 10.1007/s12253-009-9168-7
- Dourou V, 2006, ACTA HISTOCHEM, V108, P143
- Duvvuri U, 2009, CURR PROB SURG, V46, P114, DOI [10.1067/j.cpsurg.2008.10.002, 10.1067/j.cpsurg.2008.10.001]
- Gernhardt CR, 2001, J ORAL REHABIL, V28, P814, DOI 10.1046/j.1365-2842.2001.00758.x
- Grotz K. A., 1997, Strahlentherapie und Onkologie, V173, P668
- Hansen HJ, 2012, ORAL SURG ORAL MED O, V114, P50
- Hutton MF, 1974, ORAL SURG ORAL MED O, V42, P2581
- KALNINS V, 1954, J DENT RES, V33, P389, DOI 10.1177/00220345540330031201
- Kataoka SH, 2012, J ENDODONT, V38, P148, DOI 10.1016/j.joen.2011.09.022
- Kataoka SHH, 2011, J ENDODONT, V37, P1197, DOI 10.1016/j.joen.2011.05.038
- Kielbassa AM, 1997, EUR J ORAL SCI, V105, P444
- Kielbassa AM, 2006, LANCET ONCOL, V7, P326, DOI 10.1016/S1470-2045(06)70658-1
- Kielbassa Andrej M, 2002, J Clin Dent, V13, P104
- KIM S, 1985, J ENDODONT, V11, P465, DOI 10.1016/S0099-2399(85)80219-3
- KIM S, 1989, J ENDODONT, V15, P404
- Lieshout HF, 2013, CLIN ORAL INVEST, V18, P17
- MEYER I, 1962, Oral Surg Oral Med Oral Pathol, V15, P1098, DOI 10.1016/0030-4220(62)90307-9
- Mjor IA, 2001, QUINTESSENCE INT, V32, P427
- Murakami M, 2012, J ENDODONT, V38, P920, DOI 10.1016/j.joen.2012.01.010
- PIOCH T, 1992, ENDOD DENT TRAUMATOL, V8, P241
- Silva ARS, 2009, CARIES RES, V43, P43, DOI 10.1159/000192799
- Silva ARS, 2010, SUPPORT CARE CANCER, V18, P83, DOI 10.1007/s00520-009-0633-3
- Springer IN, 2005, ORAL ONCOL, V41, P723, DOI 10.1016/j.oraloncology.2005.03.011
- Trubiani O, 2003, J DENT RES, V82, P742
- Vargas PA, 2002, ORAL DIS, V8, P111, DOI 10.1034/j.1601-0825.2002.1c769.x
- Vissink A, 2003, CRIT REV ORAL BIOL M, V14, P213
- Warnakulasuriya S, 2009, ORAL ONCOL, V45, P309, DOI 10.1016/j.oraloncology.2008.06.002
- Yoshida H, 1999, J ORAL PATHOL MED, V28, P289