Smqnr VARIANTS IN CLINICAL ISOLATES OF Stenotrophomonas maltophilia IN BRAZIL

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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorGRACIA-PAEZ, Jorge Isaac
dc.contributor.authorFERRAZ, Juliana Rosa
dc.contributor.authorSILVA, Ivan Avelino Franca E
dc.contributor.authorROSSI, Flavia
dc.contributor.authorLEVIN, Anna Sara
dc.contributor.authorCOSTA, Silvia Figueiredo
dc.date.accessioned2014-01-28T22:22:10Z
dc.date.available2014-01-28T22:22:10Z
dc.date.issued2013
dc.description.abstractStenotrophomonas maltophilia contains a novel chromosomally-encoded qnr gene named Smqnr that contributes to low intrinsic resistance to quinolone. We described Smqnr in 13 clinical isolates of S. maltophilia from two Brazilian hospitals, over a 2-year period. The strains were identified by API 20 NE (bioMerieux, France). Susceptibility by microdilution method to trimetroprim/sulfamethoxazole, ciprofloxacin, levofloxacin, minocycline, ceftazidime, chloramphenicol and ticarcillin/clavulanate was performed according to CLSI. PCR detection of Smqnr gene was carried out. The sequence of Smqnr was compared with those deposited in GenBank. Pulsed-field gel electrophoresis (PFGE) of all strains was performed. Thirteen Smqnr positives isolates were sequenced and three novel variants of Smqnr were identified. All 13 Smqnr isolates had distinguishable patterns by PFGE. This is the first report of Smqnr in S. maltophilia isolated in Brazil.
dc.description.abstractS. maltophilia contem um novo gene qnr cromossômico denominado Smqnr que contribui para baixa resistência intrínseca a quinolonas. Descrevemos Smqnr em 13 isolados clínicos de S. maltophilia de dois hospitais brasileiros, ao longo do período de dois anos. Os isolados foram identificados pela API 20 NE (bioMérieux, França). Susceptibilidade pelo método de microdiluição dos seguintes antibióticos trimetroprim/sulfametoxazol, ciprofloxacina, levofloxacina, minociclina, ceftazidima, cloranfenicol e ticarcilina/clavulanato foi realizada segundo o CLSI. Detecção do gene de Smqnr foi realizada por PCR. A sequência de Smqnr foi comparada com aquelas depositadas no GenBank. Foi realizada eletroforese em gel de campo pulsado (PFGE) de todos os isolados. Treze isolados contendo Smqnr foram sequenciados e identificados três variantes do gene Smqnr. Todos os 13 isolados de Smqnr apresentaram diferentes padrões por PFGE. Este é o primeiro relato de Smqnr em isolados de S. maltophilia no Brasil
dc.description.indexMEDLINE
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [09/02281-1]
dc.identifier.citationREVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.55, n.6, p.417-420, 2013
dc.identifier.doi10.1590/S0036-46652013000600008
dc.identifier.issn0036-4665
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/4078
dc.language.isoeng
dc.publisherINST MEDICINA TROPICAL SAO PAULO
dc.relation.ispartofRevista do Instituto de Medicina Tropical de Sao Paulo
dc.rightsopenAccess
dc.rights.holderCopyright INST MEDICINA TROPICAL SAO PAULO
dc.subjectStenotrophomonas maltophilia
dc.subjectLevofloxacin resistance
dc.subjectqnr genes
dc.subject.otherresistance
dc.subject.othergenes
dc.subject.otherenterobacteriaceae
dc.subject.otherprevalence
dc.subject.otheremergence
dc.subject.otherfamily
dc.subject.wosTropical Medicine
dc.titleSmqnr VARIANTS IN CLINICAL ISOLATES OF Stenotrophomonas maltophilia IN BRAZIL
dc.title.alternativeVariantes de Smqnr de isolados clínicos de Stenotrophomonas maltophilia no Brasil
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalGRACIA-PAEZ, Jorge Isaac:Univ Sao Paulo, Fac Med, Dept Doencas Infecciosas & Parasitarias, LIM 54, BR-05403000 Sao Paulo, Brazil
hcfmusp.author.externalSILVA, Ivan Avelino Franca E:Hosp Canc AC Camargo, Serv Controle Infeccao Hosp, BR-01509010 Sao Paulo, Brazil
hcfmusp.citation.scopus5
hcfmusp.contributor.author-fmusphcJULIANA FERRAZ ROSA
hcfmusp.contributor.author-fmusphcFLAVIA ROSSI
hcfmusp.contributor.author-fmusphcANNA SARA SHAFFERMAN LEVIN
hcfmusp.contributor.author-fmusphcSILVIA FIGUEIREDO COSTA
hcfmusp.description.beginpage417
hcfmusp.description.endpage420
hcfmusp.description.issue6
hcfmusp.description.volume55
hcfmusp.origemWOS
hcfmusp.origem.pubmed24213195
hcfmusp.origem.scieloSCIELO:S0036-46652013000600417
hcfmusp.origem.scopus2-s2.0-84887389788
hcfmusp.origem.wosWOS:000326974700008
hcfmusp.publisher.citySAO PAULO
hcfmusp.publisher.countryBRAZIL
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hcfmusp.remissive.sponsorshipFAPESP
hcfmusp.scopus.lastupdate2024-05-10
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