Immunofluorescence assay for diagnosis of strongyloidiasis in immunocompromised patients

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Arquivos
art_GOTTARDI_Immunofluorescence_assay_for_diagnosis_of_strongyloidiasis_in_immunocompromised_2015.PDF (317.54 KB)
Citações na Scopus
8
Tipo de produção
article
Data de publicação
2015
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
INFORMA HEALTHCARE
Autores
CHIEFFI, Pedro Paulo
Citação
INFECTIOUS DISEASES, v.47, n.8, p.550-554, 2015
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background: Strongyloides stercoralis is a parasite that causes human strongyloidiasis. The disease ranges from asymptomatic to severe forms, which are often fatal in immunocompromised individuals. Laboratory diagnosis is challenging owing to limitations in the use of conventional parasitological techniques. The present study aimed to evaluate the indirect immunofluorescence assay (IFA) using infective larvae of S. venezuelensis as an antigen for the immunodiagnosis of human strongyloidiasis in immunocompromised patients. Methods: Serum and stool samples from 200 immunocompromised patients (HIV-positive, HTLV-1-positive, and renal, liver, and/or bone marrow transplantation candidates) were used. Stool samples were examined using three parasitological methods: Lutz, Rugai, and culture agar plate. IFA was performed using sections of infective larvae of S. venezuelensis as antigens, and showed 95.4% sensitivity and 95.8% and specificity. Results: Among the 200 patients, 17 (8.5%) were positive for S. stercoralis by at least one parasitological method, and 43 (21.5%) were positive by IFA. Conclusions: IFA can be used as a screening method for the detection of S. stercoralis in immunocompromised patients.
Palavras-chave
Strongyloides stercoralis, immunosuppression, immunodiagnosis, indirect immunofluorescence antibody
URI
https://observatorio.fm.usp.br/handle/OPI/11673
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/06
Artigos e Materiais de Revistas Científicas - LIM/37
Artigos e Materiais de Revistas Científicas - LIM/47
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