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Title: | Frequency of Plasmid-Mediated AmpC beta-Lactamases in Escherichia coli Isolates from Urine Samples in Sao Paulo, Brazil |
Authors: | ROCHA, Darlan Augusto Costa; CAMPOS, Juliana Coutinho; PASSADORE, Lilian Ferri; SAMPAIO, Suely Carlos Ferreira; NICODEMO, Antonio Carlos; SAMPAIO, Jorge Luiz Mello |
Citation: | MICROBIAL DRUG RESISTANCE, v.22, n.4, p.321-327, 2016 |
Abstract: | Plasmid-mediated AmpC beta-lactamases (PMACBLs) in Enterobacteriaceae encode resistance to third-generation cephalosporins, and these can mediate carbapenem resistance when associated with porin loss. However, no standardized phenotypic method is available for detecting these enzymes in the clinical microbiology laboratory. Limited data are available concerning the frequency of PMACBLs in Enterobacteriaceae in Brazil. This study was conducted in response to an increased cefoxitin (CFO) resistance rate of 3.7% in Escherichia coli isolates from urine samples from patients with suspected urinary tract infections during 2010. We collected 2,266 E. coli isolates prospectively during January 2012. A total of 109 (4.8%) isolates were nonsusceptible to CFO. These strains were further examined using multiplex PCR for the presence of genes encoding PMACBLs and using inhibitor assays with CFO and ceftazidime (CAZ) disks with and without phenylboronic acid. Pulsed-field gel electrophoresis was used to evaluate clonal dissemination. Genes encoding PMACBLs were detected in 1.8% of the isolates from inpatients and 0.46% of isolates from outpatients. The most prevalent gene was bla(CMY-2) and bla(CMY-4) was also detected. The phenotypic analysis showed 100% sensitivity and specificity for CMY-2 and CMY-4 when CFO-resistant isolates with a minimum zone diameter difference of 5mm for CAZ or CAZ and CFO were considered positive. Although most of the isolates were nonclonal, one clonal group with two isolates was observed. Thus, the most frequent PMACBL in E. coli from Sao Paulo, Brazil is CMY-2, and both clonal and plasmid-mediated dissemination occur. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MIP Artigos e Materiais de Revistas Científicas - HU Artigos e Materiais de Revistas Científicas - LIM/46 |
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