The recombinant LIC10508 is a plasma fibronectin, plasminogen, fibrinogen and C4BP-binding protein of Leptospira interrogans

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art_SIQUEIRA_The_recombinant_LIC10508_is_a_plasma_fibronectin_plasminogen_2016.PDF (1.65 MB)
Citações na Scopus
12
Tipo de produção
article
Data de publicação
2016
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
OXFORD UNIV PRESS
Autores
SIQUEIRA, Gabriela H.
TEIXEIRA, Aline F.
FERNANDES, Luis G.
SOUZA, Gisele O. de
ROMERO, Eliete C.
VASCONCELLOS, Silvio A.
VIEIRA, Monica L.
NASCIMENTO, Ana Lucia T. O.
Citação
PATHOGENS AND DISEASE, v.74, n.2, article ID UNSP ftv118, 11p, 2016
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Leptospirosis is a zoonosis caused by pathogenic Leptospira spp. In this study, we report that the recombinant proteins LIC10507, LIC10508 and LIC10509 are recognized by confirmed leptospirosis serum samples at both phases of the disease. The recombinant rLIC10508 and rLIC10507 are plasminogen (PLG)-binding proteins, capable of generating plasmin in the presence of a PLG activator. The proteins bind to PLG in a dose-dependent and saturable manner, fulfilling host-ligand interaction. Furthermore, rLIC10508 interacts with fibrinogen (Fg), plasma fibronectin and C4b binding protein (C4BP). The binding of rLIC10508 to Fg decreases the fibrin clotting in a thrombin-catalyzed reaction. The incubation with 4 mu M of protein promoted 40% inhibition upon clotting formation. C4BP bound to rLIC10508 retained its cofactor activity for factor I promoting the cleavage of C4b protein, which may reduce the membrane attack complex formation. Although these proteins have high amino acid sequence similarity, rLIC10508 is the most talented of the three, a behavior that might be explained by its unique putative 3D structure, whereas structures of rLIC10507 and rLIC10509 are very similar. Plasmin generation (rLIC10507 and rLIC10508), together with decreasing fibrin clot formation (rLIC10508) and impairment of the complement system (rLIC10508) may help the bacteria to overcome host defense, facilitating the infection process.
Palavras-chave
Leptospira, leptospirosis, plasmin, fibrinogen, C4BP, host interactions
URI
https://observatorio.fm.usp.br/handle/OPI/14598
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Coleções
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/49