Stochastic model for gene transcription on Drosophila melanogaster embryos

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art_PRATA_Stochastic_model_for_gene_transcription_on_Drosophila_melanogaster_2016.PDF (592.8 KB)
Citações na Scopus
8
Tipo de produção
article
Data de publicação
2016
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
AMER PHYSICAL SOC
Autores
PRATA, Guilherme N.
HORNOS, Jose Eduardo M.
Citação
PHYSICAL REVIEW E, v.93, n.2, article ID 22403, 10p, 2016
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
We examine immunostaining experimental data for the formation of stripe 2 of even-skipped (eve) transcripts on D. melanogaster embryos. An estimate of the factor converting immunofluorescence intensity units into molecular numbers is given. The analysis of the eve dynamics at the region of stripe 2 suggests that the promoter site of the gene has two distinct regimes: an earlier phase when it is predominantly activated until a critical time when it becomes mainly repressed. That suggests proposing a stochastic binary model for gene transcription on D. melanogaster embryos. Our model has two random variables: the transcripts number and the state of the source of mRNAs given as active or repressed. We are able to reproduce available experimental data for the average number of transcripts. An analysis of the random fluctuations on the number of eves and their consequences on the spatial precision of stripe 2 is presented. We show that the position of the anterior or posterior borders fluctuate around their average position by similar to 1% of the embryo length, which is similar to what is found experimentally. The fitting of data by such a simple model suggests that it can be useful to understand the functions of randomness during developmental processes.
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URI
https://observatorio.fm.usp.br/handle/OPI/16375
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Coleções
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - LIM/24