Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats
Carregando...
Citações na Scopus
7
Tipo de produção
article
Data de publicação
2013
Título da Revista
ISSN da Revista
Título do Volume
Editora
ACTA CIRURGICA BRASILEIRA
Autores
ALMEIDA, Otavio Machado de
OKSMAN, Denis
JORGETTI, Camilo
Citação
ACTA CIRURGICA BRASILEIRA, v.28, n.1, p.66-71, 2013
Resumo
PURPOSE: To compare three sterilization methods (autoclave, gamma irradiation and ethylene oxide) over non demineralized lyophilized bone allografts. METHODS: Bone allografts were implanted on paravertebral muscles of 21 rats. After 30 days animals were sacrificed and grafts underwent comparative analysis regarding histomorphometric and macroscopic parameters. RESULTS: Allografts that underwent the three sterilization methods presents similar weight gain, cortical thickness similar to control group, and less fibrosis than the control group. Grafts that underwent sterilization in autoclave presented less presence of multinucleated giant cells, although not statistically significant. There was also no statistically significant difference regarding mineralization on the three groups. CONCLUSION: The three sterilization methods cause similar effects on bone allografts regarding macroscopic and histomorphometric parameters.
Palavras-chave
Bone and Bones, Homologous, Osteogenesis, Sterilization, Rats
Referências
- Azar FM, 2009, CLIN SPORT MED, V28, P191, DOI 10.1016/j.csm.2008.10.003
- BURCHARDT H, 1987, ORTHOP CLIN N AM, V18, P187
- Calvo R, 2011, REV MED CHILE, V139, P660, DOI /S0034-98872011000500015
- CHALMERS J, 1959, J BONE JOINT SURG BR, V41, P160
- Eastlund Ted, 2006, Cell and Tissue Banking, V7, P147, DOI 10.1007/s10561-006-0003-z
- Endres S, 2009, J MUSCULOSKEL NEURON, V9, P25
- GLOWACKI J, 1986, CALCIFIED TISSUE INT, V39, P97, DOI 10.1007/BF02553297
- HAMSON KR, 1995, CALCIFIED TISSUE INT, V57, P64, DOI 10.1007/BF00298999
- KATZ J, 2010, J LONG-TERM EFF MED, V20, P271
- Kim S, 2011, J KOREAN NEUROSURG S, V50, P503, DOI 10.3340/jkns.2011.50.6.503
- KOMENDER J, 1991, CLIN ORTHOP RELAT R, P38
- MUNTING E, 1988, ACTA ORTHOP SCAND, V59, P34, DOI 10.3109/17453678809149340
- Tessier P, 1967, Ann Chir Plast, V12, P273
- Zasacki W, 1991, CLIN ORTHOP RELAT R, V72, P778
- Zhou ZK, 2011, ACTA ORTHOP BELG, V77, P670