Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/17060
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | ALMEIDA JR., Joao N. de | - |
dc.contributor.author | SZTAJNBOK, Jaques | - |
dc.contributor.author | SILVA JUNIOR, Afonso Rafael da | - |
dc.contributor.author | VIEIRA, Vinicius Adriano | - |
dc.contributor.author | GALASTRI, Anne Layze | - |
dc.contributor.author | BISSOLI, Leandro | - |
dc.contributor.author | LITVINOV, Nadia | - |
dc.contributor.author | NEGRO, Gilda Maria Barbaro Del | - |
dc.contributor.author | MOTTA, Adriana Lopes | - |
dc.contributor.author | ROSSI, Flavia | - |
dc.contributor.author | BENARD, Gil | - |
dc.date.accessioned | 2016-12-20T16:37:09Z | - |
dc.date.available | 2016-12-20T16:37:09Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | MEDICAL MYCOLOGY, v.54, n.8, p.885-889, 2016 | - |
dc.identifier.issn | 1369-3786 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/17060 | - |
dc.description.abstract | Moulds and arthroconidial yeasts are potential life-threatening agents of fungemia in immunocompromised patients. Fast and accurate identification (ID) of these pathogens hastens initiation of targeted antifungal therapy, thereby improving the patients' prognosis. We describe a new strategy that enabled the identification of moulds and arthroconidial yeasts directly from positive blood cultures by MALDI-TOFmass spectrometry (MS). Positive blood cultures (BCs) with Gram staining showing hyphae and/or arthroconidia were prospectively selected and submitted to an in-house protein extraction protocol. Mass spectra were obtained by Vitek MS (TM) system, and identifications were carried out with in the research use only (RUO) mode with an extended database (SARAMIS (TM) [v.4.12] plus in-house database). Fusarium solani, Fusarium verticillioides, Exophiala dermatitidis, Saprochaete clavata, and Trichosporon asahii had correct species ID by MALDI-TOF MS analysis of positive BCs. All cases were related to critically ill patients with high mortality fungemia and direct ID from positive BCs was helpful for rapid administration of targeted antifungal therapy. | - |
dc.language.iso | eng | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.relation.ispartof | Medical Mycology | - |
dc.rights | restrictedAccess | - |
dc.subject | Mould | - |
dc.subject | athroconidial yeasts | - |
dc.subject | fungemia | - |
dc.subject | blood culture | - |
dc.subject | MALDI-TOF mass spectrometry | - |
dc.subject.other | desorption ionization-time | - |
dc.subject.other | stream infections | - |
dc.subject.other | malignancies | - |
dc.subject.other | sepsityper | - |
dc.subject.other | candida | - |
dc.subject.other | assay | - |
dc.subject.other | ms | - |
dc.title | Rapid identification of moulds and arthroconidial yeasts from positive blood cultures by MALDI-TOF mass spectrometry | - |
dc.type | article | - |
dc.rights.holder | Copyright OXFORD UNIV PRESS | - |
dc.identifier.doi | 10.1093/mmy/myw044 | - |
dc.identifier.pmid | 27317582 | - |
dc.subject.wos | Infectious Diseases | - |
dc.subject.wos | Mycology | - |
dc.subject.wos | Veterinary Sciences | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | BISSOLI, Leandro:Univ Sao Paulo, Inst Med Trop, Lab Bacteriol LIM54, BR-05508 Sao Paulo, Brazil | - |
hcfmusp.description.beginpage | 885 | - |
hcfmusp.description.endpage | 889 | - |
hcfmusp.description.issue | 8 | - |
hcfmusp.description.volume | 54 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | 2-s2.0-84994644351 | - |
hcfmusp.origem.id | WOS:000384204300013 | - |
hcfmusp.publisher.city | OXFORD | - |
hcfmusp.publisher.country | ENGLAND | - |
hcfmusp.relation.reference | Bidart M, 2015, J CLIN MICROBIOL, V53, P1761, DOI 10.1128/JCM.03600-14 | - |
hcfmusp.relation.reference | Chitasombat MN, 2012, J INFECTION, V64, P68, DOI 10.1016/j.jinf.2011.11.002 | - |
hcfmusp.relation.reference | Clinical Laboratory Standards Institute (CLSI), 2008, M27A3 CLSI NAT COMM | - |
hcfmusp.relation.reference | Clinical and Laboratory Standards Institute (CLSI), 2008, M38A2 CLSI NAT COMM | - |
hcfmusp.relation.reference | Drancourt M, 2010, CLIN MICROBIOL INFEC, V16, P1620, DOI 10.1111/j.1469-0691.2010.03290.x | - |
hcfmusp.relation.reference | Girmenia C, 2005, J CLIN MICROBIOL, V43, P1818, DOI 10.1128/JCM.43.4.1818-1828.2005 | - |
hcfmusp.relation.reference | Gorton RL, 2014, MYCOSES, V57, P592, DOI 10.1111/myc.12205 | - |
hcfmusp.relation.reference | Huang AM, 2013, CLIN INFECT DIS, V57, P1237, DOI 10.1093/cid/cit498 | - |
hcfmusp.relation.reference | Jorgensen J, MANUAL CLIN MICROBIO | - |
hcfmusp.relation.reference | Miceli MH, 2011, LANCET INFECT DIS, V11, P142, DOI 10.1016/S1473-3099(10)70218-8 | - |
hcfmusp.relation.reference | Nalim FA, 2011, MYCOLOGIA, V103, P1302, DOI 10.3852/10-307 | - |
hcfmusp.relation.reference | Nucci M, 2014, CLIN MICROBIOL INFEC, V20, P580, DOI 10.1111/1469-0691.12409 | - |
hcfmusp.relation.reference | Nucci M, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0087784 | - |
hcfmusp.relation.reference | Nucci M, 2007, CLIN MICROBIOL REV, V20, P695, DOI 10.1128/CMR.00014-07 | - |
hcfmusp.relation.reference | Pappas PG, 2016, CLIN INFECT DIS, V62, pE1, DOI 10.1093/cid/civ933 | - |
hcfmusp.relation.reference | Pfaller MA, 2016, MED MYCOL, V54, P1, DOI 10.1093/mmy/myv076 | - |
hcfmusp.relation.reference | Spanu T, 2012, J CLIN MICROBIOL, V50, P176, DOI 10.1128/JCM.05742-11 | - |
hcfmusp.relation.reference | Sugita T, 2002, J CLIN MICROBIOL, V40, P1826, DOI 10.1128/JCM.40.5.1826-1830.2002 | - |
hcfmusp.relation.reference | Suzuki K, 2010, EUR J HAEMATOL, V84, P441, DOI 10.1111/j.1600-0609.2010.01410.x | - |
hcfmusp.relation.reference | van Belkum A, 2015, EXPERT REV PROTEOMIC, V12, P595, DOI 10.1586/14789450.2015.1091731 | - |
hcfmusp.relation.reference | Vaux S, 2014, MBIO, V5, DOI 10.1128/mBio.02309-14 | - |
hcfmusp.relation.reference | White TJ, 1990, PCR PROTOCOLS GUIDE, V18, P315, DOI 10.1139/B07-071 | - |
dc.description.index | MEDLINE | - |
dc.identifier.eissn | 1460-2709 | - |
hcfmusp.citation.scopus | 32 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MDT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - HC/ICr Artigos e Materiais de Revistas Científicas - LIM/03 Artigos e Materiais de Revistas Científicas - LIM/53 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_ALMEIDA JR._Rapid_identification_of_moulds_and_arthroconidial_yeasts_from_2016.PDF Restricted Access | publishedVersion (English) | 296.25 kB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.