Phylogenetic analysis and subgenotypic distribution of the hepatitis B virus in Recife, Brazil

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art_PINHO_Phylogenetic_analysis_and_subgenotypic_distribution_of_the_hepatitis_2013.PDF (633.82 KB)
Citações na Scopus
17
Tipo de produção
article
Data de publicação
2013
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER SCIENCE BV
Autores
MOURA, Izolda Fernandes
LOPES, Edmundo Pessoa
Citação
INFECTION GENETICS AND EVOLUTION, v.14, p.195-199, 2013
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
The analysis of the genomes of the hepatitis B virus in human hosts identifies phylogenetic variants called viral genotypes. Indeed, clinical and epidemiological observations suggest that differences in viral genotypes lead to distinct biological and clinical behaviors. The aim of this study was to evaluate the subgenotypic distribution and to conduct a phylogenetic analysis by Bayesian method of the hepatitis B virus (HBV) in patients from Recife, Brazil. From July 2009 to December 2010, 60 HBV infected patients were examined, 39 (65%) were males, whose mean age was 50 years old. 33 (55%) were genotyped by obtaining and amplifying a 1306 bp fragment comprising part of the DNA polymerase and the surface antigen of the HBV. The sequencing was performed on an ABI 3500 Automatic Sequencer and the consensus sequences were obtained by aligning both the sequenced strands (clockwise and anti-clockwise) using SEQUENCHER software. Phylogenetic analysis was conducted using the Markov Chain Monte Carlo simulation implemented by Bayesian evolutionary method by sampling trees. The following subgenotypic distribution was observed: A1 (79%), F2a (12%), A2 (6%) and F4 (3%) as was that those identified as subgenotype A1 were in the same cluster in the phylogenetic tree. In this study, the majority of patients presented the A1 subgenotype from the same viral strain. As per the distribution in the phylogenetic tree by Bayesian method, possibly this subgenotype was in the genetic make-up of Africans brought in centuries past to Brazil as slaves.
Palavras-chave
HBV genotype, HBV subgenotype, Phylogenetic analysis, Bayesian analysis, Hepatitis B virus
URI
https://observatorio.fm.usp.br/handle/OPI/1948
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MGT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/07
Artigos e Materiais de Revistas Científicas - ODS/03