Seroprevalence of toxoplasmosis, toxocariasis and cysticercosis in a rural settlement, Sao Paulo State, Brazil

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art_FERREIRA_Seroprevalence_of_toxoplasmosis_toxocariasis_and_cysticercosis_in_a_2013.PDF (256 KB)
Citações na Scopus
24
Tipo de produção
article
Data de publicação
2013
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
MANEY PUBLISHING
Autores
PRESTES-CARNEIRO, Luiz Euribel
RUBINSKY-ELEFANT, Guita
ARAUJO, Patricia Regina
TROIANI, Charlene
ZAGO, Sueli Cristina
KAIAHARA, Marcia
SASSO, Leticia
VAZ, Adelaide
Citação
PATHOGENS AND GLOBAL HEALTH, v.107, n.2, p.88-95, 2013
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background: The goal of this study was to estimate the seroprevalence of Toxocara spp., Toxoplasma gondii, and Taenia solium metacestode infection and determine some of the associated risk factors for people living in the Dona Carmen settlement, Pontal of Paranapanema, Sao Paulo, Brazil. Methods: Serum samples from 194 subjects were tested and participants answered a questionnaire. An enzyme-linked immunosorbent assay (ELISA) system based on Toxocara spp. excretory-secretory antigens obtained from the cultured second-stage larvae of Toxocara canis or vesicular fluid (VF) antigen from Taenia crassiceps metacestode was used to detect anti-Toxocara spp. IgG and IgE and anti-T. solium metacestode, respectively. For cysticercosis, the reactive ELISA samples were assayed by Western blotting using 18 kDa and 14 kDa proteins purified from VF. For T. gondii-specific IgG and IgM antibodies, anti-SAG-1, GRA-1, and GRA-7 epitope specificity was determined by ELISA. Results: Toxoplasma gondii IgG antibodies were found in 102/194 individuals (52.6%) with increased infections in females (P=0.02) and those with <= US$300 monthly income (P=0.01). Positive IgM antibodies were detected in 21/194 individuals (10.8%). Antibodies specific to Toxocara spp. were found in 28/194 subjects (14.4%). All the individuals with Toxocara spp. also had T. gondii-specific IgG antibodies. Taenia solium metacestode antibodies were detected in 11 subjects (5.7%), but none were reactive based on Western blotting. Conclusion: In spite of environmental, educational, and socioeconomic factors favoring parasite infection, the seropositivity rates of T. gondii, Toxocara spp., and T. solium metacestode-specific IgG antibodies are similar to the rates found in studies conducted in different populations in Brazil.
Palavras-chave
Toxocara spp., Toxoplasmosis, Cysticercosis, Seroprevalence, Rural settlement, Brazil
URI
https://observatorio.fm.usp.br/handle/OPI/1968
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - LIM/48