Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis

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art_BONI_Efficiency_of_noninvasive_sampling_methods_swab_together_with_2017.PDF (179.95 KB)
Citações na Scopus
19
Tipo de produção
article
Data de publicação
2017
DOI
SciELO
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
INST MEDICINA TROPICAL SAO PAULO
Autores
OYAFUSO, Luiza Keiko
SOLER, Rita de Cassia
Citação
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.59, article ID UNSP e38, 7p, 2017
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Traditional diagnostic methods used to detect American Tegumentary Leishmaniasis, such as histopathology using biopsy samples, culture techniques, and direct search for parasites, have low sensitivity and require invasive collection procedures. This study evaluates the efficiency of noninvasive sampling methods (swab) along with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis using skin and mucous samples from 25 patients who had tested positive for leishmaniasis. The outcome of the tests performance on swab samples was compatible with PCR results on biopsy samples. The findings have also shown that PCR-kDNA test is more efficient than PCR-HSP70 and qPCR tests (sensitivity of 92.3%, 40.7%, and 41%, respectively). Given the high sensitivity of the tests and the fact that the sampling method using swabs affords greater patient comfort and safety, it could be said that this method is a promising alternative to conventional biopsy-based methods for the molecular diagnosis of leishmaniasis.
Palavras-chave
Tegumentary Leishmaniasis, Polymerase Chain Reaction, Swab, Leishmania braziliensis
URI
https://observatorio.fm.usp.br/handle/OPI/21599
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Coleções
Artigos e Materiais de Revistas Científicas - LIM/38