Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/21968
Title: | Validation of QF-PCR for prenatal diagnoses in a Brazilian population |
Authors: | MORAES, Renata Wendel de; CARVALHO, Mario Henrique Burlacchini de; AMORIM-FILHO, Antonio Gomes de; FRANCISCO, Rossana Pulcineli Vieira; ROMAO, Renata Moscolini; LEVI, Jose Eduardo; ZUGAIB, Marcelo |
Citation: | CLINICS, v.72, n.7, p.400-404, 2017 |
Abstract: | OBJECTIVES: Quantitative fluorescence polymerase chain reaction (QF-PCR) is a rapid and reliable method for screening aneuploidies, but in Brazil, it is not used in public services. We investigated the accuracy of QF-PCR for the prenatal recognition of common aneuploidies and compared these results with cytogenetic results in our laboratory. METHOD: A ChromoQuant QF-PCR kit containing 24 primer pairs targeting loci on chromosomes 21, 13, 18, X and Y was employed to identify aneuploidies of the referred chromosomes. RESULTS: A total of 162 amniotic fluid samples analyzed using multiplex QF-PCR were compared with karyotyping analysis. The QF-PCR results were consistent with the results of cytogenetic analysis in 95.4% of all samples. CONCLUSION: QF-PCR was demonstrated to be efficient and reliable for prenatal aneuploidy screening. This study suggests that QF-PCR can be used as a rapid diagnostic method. However, rearrangements and some mosaic samples cannot be detected with this test; thus, those exceptions must undergo cytogenetic analysis. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MOG Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - HC/InCor Artigos e Materiais de Revistas Científicas - LIM/52 Artigos e Materiais de Revistas Científicas - LIM/57 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_MORAES_Validation_of_QFPCR_for_prenatal_diagnoses_in_a_2017.PDF | publishedVersion (English) | 175.62 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.