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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | DURAN, Adriana Feliciano Alves | - |
dc.contributor.author | NEVES, Luana de Paiva | - |
dc.contributor.author | SILVA, Flavia Ribeiro Santos da | - |
dc.contributor.author | MACHADO, Gabriel Capella | - |
dc.contributor.author | FERREIRA, Graziele Cristina | - |
dc.contributor.author | LOURENCO, Juliana D. | - |
dc.contributor.author | TANAKA, Aparecida Sadae | - |
dc.contributor.author | MARTINS, Milton de Arruda | - |
dc.contributor.author | LOPES, Fernanda D. T. Q. S. | - |
dc.contributor.author | SASAKI, Sergio Daishi | - |
dc.date.accessioned | 2018-09-13T15:22:32Z | - |
dc.date.available | 2018-09-13T15:22:32Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.111, p.1214-1221, 2018 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/28001 | - |
dc.description.abstract | Protease/anti-protease imbalance is the main pathogenic mechanism of emphysema and protease inhibitors have been recognized as potential molecules to treat the disease conditions. In this work the rBmTI-6 first domain (rBmTI-6-D1), a recombinant Kunitz-type serine proteinase inhibitor, was used to verify its effect in prevention or minimization of PPE-induced emphysema in mice. C57BL/6 mice were submitted to a PPE-induced emphysema model and treated with rBmTI-6-D1 before the emphysema development. We showed that the rBmTI-6D1 treatment was sufficient to avoid the loss of elastic recoil, an effective decrease in alveolar enlargement and in the number of macrophages and lymphocytes in bronchoalveolar lavage fluid. Proteolytic analysis showed a significant increase in elastase activity in PPE-VE (induced emphysema) group that is controlled by rBmTI-6D1. Kallikrein activity was decreased in the PPE-rBmTI6 (induced emphysema and inhibitor treated) group when compared to PPE-VE group. Although rBmTI-6-D1, did not present a neutrophil elastase (NE) inhibitory activity, the results show that the inhibitor interfered in the pathway of NE secretion in PPE-emphysema mice model. The role of rBmTI-6-D1 in the prevention of emphysema development in the mice model, apparently, is related with a control of inflammatory response due the trypsin/kallikrein inhibitory activity of rBmTI-6-D1. | - |
dc.description.sponsorship | Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/07001-7] | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) | - |
dc.description.sponsorship | Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [1415647] | - |
dc.language.iso | eng | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.ispartof | International Journal of Biological Macromolecules | - |
dc.rights | restrictedAccess | - |
dc.subject | COPD | - |
dc.subject | Emphysema | - |
dc.subject | Serine protease inhibitor | - |
dc.subject | rBmTI-6 | - |
dc.subject.other | human neutrophil elastase | - |
dc.subject.other | alpha(1)-antitrypsin deficiency | - |
dc.subject.other | boophilus-microplus | - |
dc.subject.other | inhibitor | - |
dc.subject.other | disease | - |
dc.subject.other | macrophages | - |
dc.subject.other | expression | - |
dc.subject.other | tissue | - |
dc.subject.other | lungs | - |
dc.title | rBmTI-6 attenuates pathophysiological and inflammatory parameters of induced emphysema in mice | - |
dc.type | article | - |
dc.rights.holder | Copyright ELSEVIER SCIENCE BV | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2018.01.066 | - |
dc.identifier.pmid | 29339284 | - |
dc.subject.wos | Biochemistry & Molecular Biology | - |
dc.subject.wos | Chemistry, Applied | - |
dc.subject.wos | Polymer Science | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | DURAN, Adriana Feliciano Alves:UFABC, CCNH, Campus Sao Bernardo Campo Rua Arcturus 03, BR-09606070 Sao Paulo, Brazil | - |
hcfmusp.author.external | NEVES, Luana de Paiva:UFABC, CCNH, Campus Sao Bernardo Campo Rua Arcturus 03, BR-09606070 Sao Paulo, Brazil | - |
hcfmusp.author.external | SILVA, Flavia Ribeiro Santos da:UFABC, CCNH, Campus Sao Bernardo Campo Rua Arcturus 03, BR-09606070 Sao Paulo, Brazil | - |
hcfmusp.author.external | MACHADO, Gabriel Capella:UFABC, CCNH, Campus Sao Bernardo Campo Rua Arcturus 03, BR-09606070 Sao Paulo, Brazil | - |
hcfmusp.author.external | FERREIRA, Graziele Cristina:UFABC, CCNH, Campus Sao Bernardo Campo Rua Arcturus 03, BR-09606070 Sao Paulo, Brazil | - |
hcfmusp.author.external | TANAKA, Aparecida Sadae:UNIFESP EPM, Dept Bioquim, Sao Paulo, Brazil | - |
hcfmusp.author.external | SASAKI, Sergio Daishi:UFABC, CCNH, Campus Sao Bernardo Campo Rua Arcturus 03, BR-09606070 Sao Paulo, Brazil | - |
hcfmusp.description.beginpage | 1214 | - |
hcfmusp.description.endpage | 1221 | - |
hcfmusp.description.volume | 111 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000429391000137 | - |
hcfmusp.origem.id | 2-s2.0-85041627539 | - |
hcfmusp.publisher.city | AMSTERDAM | - |
hcfmusp.publisher.country | NETHERLANDS | - |
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dc.description.index | MEDLINE | - |
dc.identifier.eissn | 1879-0003 | - |
hcfmusp.citation.scopus | 6 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCM Artigos e Materiais de Revistas Científicas - LIM/20 Artigos e Materiais de Revistas Científicas - ODS/03 |
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