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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | IVANOVIC, Renato F. | - |
dc.contributor.author | VIANA, Nayara I. | - |
dc.contributor.author | MORAIS, Denis R. | - |
dc.contributor.author | MOURA, Caio | - |
dc.contributor.author | SILVA, Iran A. | - |
dc.contributor.author | LEITE, Katia R. | - |
dc.contributor.author | PONTES-JUNIOR, Jose | - |
dc.contributor.author | NAHAS, William C. | - |
dc.contributor.author | SROUGI, Miguel | - |
dc.contributor.author | REIS, Sabrina T. | - |
dc.date.accessioned | 2018-11-21T16:59:15Z | - |
dc.date.available | 2018-11-21T16:59:15Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | BMC CANCER, v.18, article ID 992, 6p, 2018 | - |
dc.identifier.issn | 1471-2407 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/29323 | - |
dc.description.abstract | BackgroundThe imbalance between the action of the tissue inhibitors of matrix metalloproteinases (TIMPs) and the matrix metalloproteinases (MMPs) is one component of metastasis physiology. TIMP-1 overrides MMP-9 activity in cancer and might be regulated by miR-618. The aims of this study were to clarify whether TIMP-1 expression is modified by miR-618 and to clarify the effect of miR-618 expression on the invasion of prostate cancer cells. We also studied miR-618 expression in surgical specimens of patients with localized prostate cancer submitted to open radical prostatectomy.MethodsAfter transfection of miR-618 or its antagonist in DU145 cells, qRT-PCR for TIMP-1/MMP-9 and both ELISA and zymography for MMP-9 were performed. Total miRNA was extracted from surgical specimens of PCa, and miR-618 expression was examined for correlations with Gleason score, pathological status and biochemical recurrence.ResultsDU145 cells transfected with miR-618 had a 76% reduction in TIMP-1 expression relative to control cells (p=0.003). miR-618 inhibition reduced MMP-9 expression by 31% (p=0.032) and MMP-9 absorbance evaluated with ELISA assay (p=0.06).Zymography suggested higher MMP-9 activity in DU145 cells transfected with miR-618 than those transfected with miR-618 inhibitor, but the difference was not significant (p=0.55). However, miR-618 expression was lower in surgical specimens of patients with Gleason score>7 (p=0.08) and more advanced disease (p=0.07).ConclusionsIn vitro, miR-618 overexpression decreases TIMP-1 and miR-618 inhibition decreases MMP-9, suggesting that miR-618 might be an oncomiR. However, the analysis of clinical samples of localized prostate cancer revealed an inconsistent pattern, as increased miR-618 expression was associated with lower Gleason score and pathological status. Further studies are needed to address whether miR-618 is a context-dependent miRNA. | - |
dc.description.sponsorship | Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2015/00845-6, 2012/21833-8] | - |
dc.language.iso | eng | - |
dc.publisher | BMC | - |
dc.relation.ispartof | BMC Cancer | - |
dc.rights | openAccess | - |
dc.subject | Prostate cancer | - |
dc.subject | MMP-9 | - |
dc.subject | TIMP-1 | - |
dc.subject | microRNA | - |
dc.subject | Invasion | - |
dc.subject.other | matrix metalloproteinases | - |
dc.subject.other | messenger-rna | - |
dc.subject.other | biomarkers | - |
dc.subject.other | inhibitors | - |
dc.subject.other | tissue | - |
dc.subject.other | prognosis | - |
dc.title | miR-618: possible control over TIMP-1 and its expression in localized prostate cancer | - |
dc.type | article | - |
dc.rights.holder | Copyright BMC | - |
dc.identifier.doi | 10.1186/s12885-018-4930-4 | - |
dc.identifier.pmid | 30340564 | |
dc.subject.wos | Oncology | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.description.articlenumber | 992 | - |
hcfmusp.description.volume | 18 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000447737100007 | - |
hcfmusp.origem.id | 2-s2.0-85055077806 | - |
hcfmusp.publisher.city | LONDON | - |
hcfmusp.publisher.country | ENGLAND | - |
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dc.description.index | MEDLINE | - |
hcfmusp.citation.scopus | 7 | - |
hcfmusp.scopus.lastupdate | 2024-04-12 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCG Artigos e Materiais de Revistas Científicas - HC/ICESP Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/55 Artigos e Materiais de Revistas Científicas - ODS/03 |
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art_IVANOVIC_miR618_possible_control_over_TIMP1_and_its_expression_2018.PDF | publishedVersion (English) | 758.12 kB | Adobe PDF | View/Open |
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