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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | MARTINS, Vanessa | - |
dc.contributor.author | TEODORO, Walcy Rosolia | - |
dc.contributor.author | VELOSA, Ana Paula Pereira | - |
dc.contributor.author | ANDRADE, Priscila | - |
dc.contributor.author | FARHAT, Cecilia | - |
dc.contributor.author | FABRO, Alexandre Todorovic | - |
dc.contributor.author | CAPELOZZI, Vera Luiza | - |
dc.date.accessioned | 2018-11-21T17:06:25Z | - |
dc.date.available | 2018-11-21T17:06:25Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | HISTOLOGY AND HISTOPATHOLOGY, v.33, n.10, p.1111-1123, 2018 | - |
dc.identifier.issn | 0213-3911 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/29595 | - |
dc.description.abstract | Anomalous histoarchitecture with increased levels of type-V collagen (Col V) in lungs of human idiopathic pulmonary fibrosis (IPF) and bleomycin (BLM) airway-centered interstitial fibrosis suggest that this collagen can be a possible trigger involved in the pathogenesis of these diseases. Butylated hydroxytoluene (BHT) injury model revealed a distal involvement of lung parenchyma with significant endothelial injury and fibrotic response, contrasting with the BLM airway-centered insult. We undertook this study to analyze whether BHT alters distal airway/alveolar epithelial cells (AECs) and extracellular matrix (ECM) signaling involved in the initiation and progression of pulmonary fibrosis in a different pathway concerning overexpression of Col V. Female mice C57BL/6 (n=6) were instilled intraperitoneally with 400mg/kg of BHT dissolved in 1 mL of corn oil and euthanized at day 14 or 21 after BHT administration. Morphometry, immunohistochemistry and transmission electron microscopy were performed to characterize microscopic and submicroscopic changes of AECs and endothelial cells through transforming growth factor beta (TGF-beta) basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) expression. Immunofluorescence and immunogold electron microscopy were performed to characterize Col V. Quantitative polymerase chain reaction (qPCR) was used to confirm differential levels of RNA messenger. BHT lungs showed marked fibrotic areas and hyperplastic AECs. The alveolar damage caused destruction of elastic fibers and a critical increase of Col V in ECM of distal lung parenchyma. Fibrogenesis-promoting markers TGF-beta, bFGF and VEGF were also overexpressed in situ, coinciding with up-regulation in remodeling enzymes, growth factors, cytokines, transduction and transcription genes. BHT alters distal lung parenchyma signaling involved in pulmonary fibrosis highlighted similarities to human IPF in a pathway involving Col V arising as a promissory model to identify effective therapeutic targets. | - |
dc.description.sponsorship | National Council for Scientific and Technological Development [CNPq-471939/2010-2, 483005/2012-6] | - |
dc.description.sponsorship | Foundation for the Support of Research of the State of Sao Paulo [FAPESP 12/03543-2, FAPESP 11/09181-2, FAPESP 2012/07040-5, FAPESP 2013/05886-7] | - |
dc.description.sponsorship | Laboratories for Medical Research [LIMs] | - |
dc.description.sponsorship | Hospital das Clinicas | - |
dc.description.sponsorship | University of Sao Paulo Medical School | - |
dc.description.sponsorship | Coordination for the Improvement of Higher Level Personnel (CAPES) | - |
dc.language.iso | eng | - |
dc.publisher | F HERNANDEZ | - |
dc.relation.ispartof | Histology and Histopathology | - |
dc.rights | restrictedAccess | - |
dc.subject | Pulmonary fibrosis | - |
dc.subject | Butylated hydroxytoluene | - |
dc.subject | Extracellular matrix | - |
dc.subject | Type-V collagen | - |
dc.subject | RT-PCR | - |
dc.subject | Immunohistochemistry | - |
dc.subject | Immunofluorescence | - |
dc.subject | Electron microscopy | - |
dc.subject.other | usual interstitial pneumonia | - |
dc.subject.other | pulmonary-fibrosis | - |
dc.subject.other | molecular-mechanisms | - |
dc.subject.other | systemic-sclerosis | - |
dc.subject.other | in-vitro | - |
dc.subject.other | bleomycin | - |
dc.subject.other | expression | - |
dc.subject.other | gene | - |
dc.subject.other | immunization | - |
dc.subject.other | diagnosis | - |
dc.title | Butylated hydroxytoluene induces type-V collagen and overexpression of remodeling genes/proteins in experimental lung fibrosis | - |
dc.type | article | - |
dc.rights.holder | Copyright F HERNANDEZ | - |
dc.identifier.doi | 10.14670/HH-18-010 | - |
dc.identifier.pmid | 29870049 | - |
dc.subject.wos | Cell Biology | - |
dc.subject.wos | Pathology | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | MARTINS, Vanessa:Univ Sao Paulo, Dept Pathol, Fac Med, Sao Paulo, Brazil | - |
hcfmusp.author.external | FARHAT, Cecilia:Univ Sao Paulo, Dept Pathol, Fac Med, Sao Paulo, Brazil | - |
hcfmusp.author.external | FABRO, Alexandre Todorovic:Univ Sao Paulo, Ribeirao Preto Med Sch, Sao Paulo, Brazil | - |
hcfmusp.description.beginpage | 1111 | - |
hcfmusp.description.endpage | 1123 | - |
hcfmusp.description.issue | 10 | - |
hcfmusp.description.volume | 33 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000443953000009 | - |
hcfmusp.origem.id | 2-s2.0-85053178724 | - |
hcfmusp.publisher.city | MURCIA | - |
hcfmusp.publisher.country | SPAIN | - |
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dc.description.index | MEDLINE | - |
dc.identifier.eissn | 1699-5848 | - |
hcfmusp.citation.scopus | 4 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCM Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/02 Artigos e Materiais de Revistas Científicas - LIM/03 Artigos e Materiais de Revistas Científicas - LIM/17 |
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