Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/29992
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | |
dc.contributor.author | DAMOUS, Luciana Lamarao | |
dc.contributor.author | CARVALHO, Ana Elisa Teofilo Saturi de | |
dc.contributor.author | NAKAMUTA, Juliana Sanajotti | |
dc.contributor.author | SHIROMA, Marcos Eiji | |
dc.contributor.author | LOUZADA, Andressa Cristina Sposato | |
dc.contributor.author | SOARES- JR., Jose Maria | |
dc.contributor.author | KRIEGER, Jose Eduardo | |
dc.contributor.author | BARACAT, Edmund C. | |
dc.date.accessioned | 2019-01-17T13:34:48Z | - |
dc.date.available | 2019-01-17T13:34:48Z | - |
dc.date.issued | 2018 | |
dc.identifier.citation | STEM CELL RESEARCH & THERAPY, v.9, article ID 323, 10p, 2018 | |
dc.identifier.issn | 1757-6512 | |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/29992 | - |
dc.description.abstract | The use of secretome may be a new strand of cell therapy, which is equal to or even superior to the injection of live cells, called cell-free therapy. In ovarian transplantation, this approach may be a therapeutic possibility for the ovarian graft in hypoxia. We designed the present study to evaluate whether the cell-free therapy with the secretome of adipose tissue-derived stem cells (ASCs) in rat frozen-thawed ovarian grafts could protect a graft against ischemic injury. A single dose of rat ASCs secretome or vehicle was injected into the bilateral frozen-thawed ovaries of 18 adult female rats immediately after an autologous transplant. Nine animals were used to control the cryopreservation protocol and were evaluated before and after the cryopreservation process. Daily vaginal smears were performed for estrous cycle evaluation until euthanasia on postoperative day 30. Follicle viability by trypan blue, graft morphology by HE, and apoptosis by TUNEL and cleaved-caspase-3 were assessed. No differences were found with respect to estrous cycle resumption and follicle viability (p > 0.05). However, compared with the vehicle-treated grafts, the morphology of the secretome-treated grafts was impaired, showing reduced follicular population and increased apoptosis (p < 0.05). ASC secretome impaired the rat frozen-thawed ovarian graft from ischemic injury. However, more studies are needed to evaluate the factors involved and the possibility of applying the secretome in scaffolds to optimize its use. | eng |
dc.description.sponsorship | Sao Paulo Research Foundation (Fundacao de Amparo a Pesquisa do EStado de Sao Paulo - FAPESP) [2010/17897-5, 2012/09469-9] | |
dc.language.iso | eng | |
dc.publisher | BMC | eng |
dc.relation.ispartof | Stem Cell Research & Therapy | |
dc.rights | openAccess | eng |
dc.subject | Fertility preservation | eng |
dc.subject | Ovarian transplantation | eng |
dc.subject | Stem cells | eng |
dc.subject | Secretome | eng |
dc.subject | Rat | eng |
dc.subject.other | fertility preservation | eng |
dc.subject.other | transplantation | eng |
dc.subject.other | cryopreservation | eng |
dc.subject.other | injury | eng |
dc.title | Cell-free therapy with the secretome of adipose tissue-derived stem cells in rats' frozen-thawed ovarian grafts | eng |
dc.type | article | eng |
dc.rights.holder | Copyright BMC | eng |
dc.identifier.doi | 10.1186/s13287-018-1054-3 | |
dc.identifier.pmid | 30463630 | |
dc.subject.wos | Cell Biology | eng |
dc.subject.wos | Medicine, Research & Experimental | eng |
dc.type.category | original article | eng |
dc.type.version | publishedVersion | eng |
hcfmusp.author.external | NAKAMUTA, Juliana Sanajotti:Univ Sao Paulo, Fac Med, Heart Inst Incor, Lab Genet & Mol Cardiol, Dr Eneas de Carvalho Aguiar Av 44,10th Floor, BR-05403000 Sao Paulo, SP, Brazil | |
hcfmusp.description.articlenumber | 323 | |
hcfmusp.description.volume | 9 | |
hcfmusp.origem | WOS | |
hcfmusp.origem.id | WOS:000450956400008 | |
hcfmusp.origem.id | 2-s2.0-85056918945 | |
hcfmusp.publisher.city | LONDON | eng |
hcfmusp.publisher.country | ENGLAND | eng |
hcfmusp.relation.reference | Blande IS, 2009, TRANSFUSION, V49, P2680, DOI 10.1111/j.1537-2995.2009.02346.x | eng |
hcfmusp.relation.reference | Chen BC, 2018, INT J MOL SCI, V19, DOI 10.3390/ijms19051298 | eng |
hcfmusp.relation.reference | Chuang TJ, 2012, J TRAUMA ACUTE CARE, V73, P1161, DOI 10.1097/TA.0b013e318265d128 | eng |
hcfmusp.relation.reference | Cosenza S, 2018, THERANOSTICS, V8, P1399, DOI 10.7150/thno.21072 | eng |
hcfmusp.relation.reference | Damous LL, 2015, REPROD BIOL ENDOCRIN, V13, DOI 10.1186/s12958-015-0104-2 | eng |
hcfmusp.relation.reference | Damous LL, 2015, J ASSIST REPROD GEN, V32, P1285, DOI 10.1007/s10815-015-0527-x | eng |
hcfmusp.relation.reference | Damous LL, 2015, STEM CELL RES THER, V6, DOI 10.1186/s13287-015-0068-3 | eng |
hcfmusp.relation.reference | Damous LL, 2014, J OVARIAN RES, V7, DOI 10.1186/1757-2215-7-34 | eng |
hcfmusp.relation.reference | Danoviz ME, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0012077 | eng |
hcfmusp.relation.reference | Danoviz ME, 2011, STEM CELLS DEV, V20, P661, DOI 10.1089/scd.2010.0231 | eng |
hcfmusp.relation.reference | Demetris AJ, 2006, AM J SURG PATHOL, V30, P986, DOI 10.1097/00000478-200608000-00009 | eng |
hcfmusp.relation.reference | Donnez J, 2013, FERTIL STERIL, V99, P1503, DOI 10.1016/j.fertnstert.2013.03.030 | eng |
hcfmusp.relation.reference | Furuhashi K, 2013, J AM SOC NEPHROL, V24, P587, DOI 10.1681/ASN.2012030264 | eng |
hcfmusp.relation.reference | Girao-Silva T, 2014, BIOMED ENG ONLINE, V13, DOI 10.1186/1475-925X-13-54 | eng |
hcfmusp.relation.reference | Gunasena KT, 1997, HUM REPROD, V12, P101, DOI 10.1093/humrep/12.1.101 | eng |
hcfmusp.relation.reference | Jadoul P, 2017, HUM REPROD, V32, P1046, DOI 10.1093/humrep/dex040 | eng |
hcfmusp.relation.reference | Khanmohammadi N, 2018, CELL J, V20, P10, DOI 10.22074/cellj.2018.4919 | eng |
hcfmusp.relation.reference | Konala VBR, 2016, CYTOTHERAPY, V18, P13, DOI 10.1016/j.jcyt.2015.10.008 | eng |
hcfmusp.relation.reference | Lauvrud AT, 2017, J TISSUE ENG REGEN M, V11, P2490, DOI 10.1002/term.2147 | eng |
hcfmusp.relation.reference | Makridakis M, 2013, BBA-PROTEINS PROTEOM, V1834, P2380, DOI 10.1016/j.bbapap.2013.01.032 | eng |
hcfmusp.relation.reference | Maumus M, 2013, BIOCHIMIE, V95, P2229, DOI 10.1016/j.biochi.2013.04.017 | eng |
hcfmusp.relation.reference | Meirow D, 2015, HUM REPROD, V30, P2453, DOI 10.1093/humrep/dev210 | eng |
hcfmusp.relation.reference | Nakamuta JS, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0006005 | eng |
hcfmusp.relation.reference | Ozcan S, 2016, AGING-US, V8, P1316, DOI 10.18632/aging.100971 | eng |
hcfmusp.relation.reference | Pfaffl MW, 2001, NUCLEIC ACIDS RES, V29, DOI 10.1093/nar/29.9.e45 | eng |
hcfmusp.relation.reference | Pires AO, 2016, STEM CELLS DEV, V25, P1073, DOI 10.1089/scd.2016.0048 | eng |
hcfmusp.relation.reference | Skalnikova HK, 2013, BIOCHIMIE, V95, P2196, DOI 10.1016/j.biochi.2013.07.015 | eng |
hcfmusp.relation.reference | Vassilieva IO, 2018, BIOCHEM BIOPH RES CO, V496, P1162, DOI 10.1016/j.bbrc.2018.01.163 | eng |
hcfmusp.relation.reference | Wallace WHB, 2016, FERTIL STERIL, V105, P6, DOI 10.1016/j.fertnstert.2015.11.041 | eng |
hcfmusp.relation.reference | Wang CF, 2017, DNA CELL BIOL, DOI 10.1089/dna.2017.3735 | eng |
hcfmusp.relation.reference | Xia HB, 2017, GENET MOL RES, V16, DOI 10.4238/gmr16039278 | eng |
hcfmusp.relation.reference | Yang JA, 2010, EXPERT OPIN BIOL TH, V10, P495, DOI 10.1517/14712591003610598 | eng |
hcfmusp.relation.reference | Zhao L, 2018, BIOMED PHARMACOTHER, V104, P672, DOI 10.1016/j.biopha.2018.05.100 | eng |
hcfmusp.relation.reference | Zhao LN, 2017, STEM CELL RES THER, V8, DOI 10.1186/s13287-017-0578-2 | eng |
dc.description.index | MEDLINE | eng |
hcfmusp.citation.scopus | 9 | - |
hcfmusp.scopus.lastupdate | 2024-04-12 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCP Artigos e Materiais de Revistas Científicas - FM/MOG Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - HC/InCor Artigos e Materiais de Revistas Científicas - LIM/13 Artigos e Materiais de Revistas Científicas - LIM/58 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_PREHAM_CD4_T_Cells_Alter_the_Stromal_Microenvironment_and_2018.PDF | publishedVersion (English) | 3.62 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.