Inflammatory gene expression analysis after prebiotic, probiotic and synbiotic supplementation in experimental nonalcoholic fatty liver disease

Imagem de Miniatura
Arquivos
art_SANTOS_Inflammatory_gene_expression_analysis_after_prebiotic_probiotic_and_2019.PDF (196.71 KB)
Citações na Scopus
3
Tipo de produção
article
Data de publicação
2019
DOI
Scopus
Web of Science
Título da Revista
ISSN da Revista
Título do Volume
Editora
EMERALD GROUP PUBLISHING LTD
Autores
RIBEIRO, Daniel Araki
FERREIRA, Jessica Almeida da Cruz
AGUIAR, Odair
ALVES, Claudia Cristina
Citação
NUTRITION & FOOD SCIENCE, v.49, n.1, p.75-84, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Purpose The purpose of this study is to evaluate the effects of prebiotic, probiotic and synbiotic supplementation on liver histopathology and TLR-4, NF kappa B and TNF-alpha gene expression involved in the inflammatory cascade and pathogenesis of experimental nonalcoholic fatty liver disease (NAFLD). Design/methodology/approach Wistar male adult rats (n = 40) were submitted to hypercholesterolemic conditions (60 days). On Day 30 of hypercholesterolemic conditions, rats were subdivided in five groups: negative control (NC), positive control (PC), prebiotic (PRE), probiotic (PRO) and synbiotic (SYN). All rats were sacrificed on Day 60. Liver tissue was used to verify histopathological changes and gene expression. Gene expression of TLR-4, TNF-alpha and NF kappa B was evaluated in liver tissue using RT-qPCR. Findings Histopathological analysis: PC showed more changes than NC, and PRE and SYN showed fewer alterations than PC. Gene expression analysis: PRE showed higher TLR-4, and NF kappa B and TNF-alpha compared to PC. Also, PRE showed higher TLR-4 when compared to PRO and SYN. SYN group revealed higher TLR-4 and NF kappa B expressions compared to PC. PRO group also showed higher NF kappa B expression compared to PC. Originality/value NAFLD is a significant health concern, and it found that prebiotic, probiotic and synbiotic supplementation could have positive effects as a nonpharmacological approach to control this disease.
Palavras-chave
Probiotic, NFkB, Nonalcoholic fatty liver disease, Prebiotic, TLR-4, TNF-
URI
https://observatorio.fm.usp.br/handle/OPI/31155
Referências
  1. Abd El-Kader SM, 2015, WORLD J HEPATOL, V7, P846, DOI 10.4254/wjh.v7.i6.846
  2. Abu-Shanab A, 2010, NAT REV GASTRO HEPAT, V7, P691, DOI 10.1038/nrgastro.2010.172
  3. Valadez JMA, 2016, THER CLIN RISK MANAG, V12, P1729, DOI 10.2147/TCRM.S115902
  4. Alisi A, 2012, FRONT CELL INFECT MI, V2, DOI 10.3389/fcimb.2012.00132
  5. Alisi A, 2010, J PEDIATR GASTR NUTR, V50, P645, DOI 10.1097/MPG.0b013e3181c7bdf1
  6. Anand G, 2016, SEMIN LIVER DIS, V36, P37, DOI 10.1055/s-0035-1571276
  7. Arslan N, 2014, WORLD J GASTROENTERO, V20, P16452, DOI 10.3748/wjg.v20.i44.16452
  8. Bakhshimoghaddam F, 2018, J NUTR, V148, P1276, DOI 10.1093/jn/nxy088
  9. Baldwin AS, 1996, ANNU REV IMMUNOL, V14, P649, DOI 10.1146/annurev.immunol.14.1.649
  10. Bigorgne AE, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0151063
  11. Bouhnik Y, 1997, J NUTR, V127, P444
  12. Capitan-Canadas F, 2014, MOL NUTR FOOD RES, V58, P1098, DOI 10.1002/mnfr.201300497
  13. Hernandez-Rodas MC, 2015, INT J MOL SCI, V16, P25168, DOI 10.3390/ijms161025168
  14. Cesaro C, 2011, DIGEST LIVER DIS, V43, P431, DOI 10.1016/j.dld.2010.10.015
  15. Chappuis E, 2017, MOLECULES, V22, DOI 10.3390/molecules22101725
  16. Compare D, 2012, NUTR METAB CARDIOVAS, V22, P471, DOI 10.1016/j.numecd.2012.02.007
  17. Delzenne NM, 2013, BRIT J NUTR, V109, pS81, DOI 10.1017/S0007114512004047
  18. Duseja A, 2014, CLIN LIVER DIS, V18, P59, DOI 10.1016/j.cld.2013.09.002
  19. Eslamparast T, 2014, AM J CLIN NUTR, V99, P535, DOI 10.3945/ajcn.113.068890
  20. Gratz SW, 2010, WORLD J GASTROENTERO, V16, P403, DOI 10.3748/wjg.v16.i4.403
  21. Hadi Amir, 2018, Crit Rev Food Sci Nutr, P1, DOI 10.1080/10408398.2018.1458021
  22. Henao-Mejia J, 2012, NATURE, V482, P179, DOI 10.1038/nature10809
  23. Iacono A, 2011, J NUTR BIOCHEM, V22, P699, DOI 10.1016/j.jnutbio.2010.10.002
  24. Ilan Y, 2012, WORLD J GASTROENTERO, V18, P2609, DOI 10.3748/wjg.v18.i21.2609
  25. John B, 2005, J IMMUNOL, V175, P1643, DOI 10.4049/jimmunol.175.3.1643
  26. Manzoni MSJ, 2008, EUR FOOD RES TECHNOL, V227, P1591, DOI 10.1007/s00217-008-0883-1
  27. Kapil S, 2016, WORLD J GASTROENTERO, V22, P9346, DOI 10.3748/wjg.v22.i42.9346
  28. Kawai T, 2006, CELL DEATH DIFFER, V13, P816, DOI 10.1038/sj.cdd.4401850
  29. Kok N, 1996, BRIT J NUTR, V76, P881, DOI 10.1079/BJN19960094
  30. Larkin TA, 2009, EUR J CLIN NUTR, V63, P238, DOI 10.1038/sj.ejcn.1602910
  31. Lawrence T, 2009, CSH PERSPECT BIOL, V1, DOI 10.1101/cshperspect.a001651
  32. Li DY, 2013, JPEN-PARENTER ENTER, V37, P787, DOI 10.1177/0148607113481623
  33. Li ZP, 2003, HEPATOLOGY, V37, P343, DOI 10.1053/jhep.2003.50048
  34. Lin P, 2015, PLOS ONE, V10, DOI 10.1371/journal.pone.0140346
  35. Loomba R, 2017, CELL METAB, V25, P1054, DOI 10.1016/j.cmet.2017.04.001
  36. Ma YY, 2013, WORLD J GASTROENTERO, V19, P6911, DOI 10.3748/wjg.v19.i40.6911
  37. Mehal WZ, 2013, NAT REV GASTRO HEPAT, V10, P637, DOI 10.1038/nrgastro.2013.146
  38. Miura K, 2014, WORLD J GASTROENTERO, V20, P7381, DOI 10.3748/wjg.v20.i23.7381
  39. Mofidi F, 2017, BRIT J NUTR, V117, P662, DOI 10.1017/S0007114517000204
  40. Mogensen TH, 2009, CLIN MICROBIOL REV, V22, P240, DOI 10.1128/CMR.00046-08
  41. Muzio M, 2000, J IMMUNOL, V164, P5998, DOI 10.4049/jimmunol.164.11.5998
  42. Parnell JA, 2012, LIVER INT, V32, P701, DOI 10.1111/j.1478-3231.2011.02730.x
  43. Paschos P, 2009, HIPPOKRATIA, V13, P9
  44. Pfaffl MW, 2001, NUCLEIC ACIDS RES, V29, DOI 10.1093/nar/29.9.e45
  45. Reeves PG, 1997, J NUTR, V127, pS838
  46. Saberi M, 2009, CELL METAB, V10, P419, DOI 10.1016/j.cmet.2009.09.006
  47. Sakaguchi S, 2011, DRUG METAB PHARMACOK, V26, P30, DOI 10.2133/dmpk.DMPK-10-RV-087
  48. Son G, 2010, GASTROENT RES PRACT, DOI 10.1155/2010/453563
  49. Sumida Y, 2018, J GASTROENTEROL, V53, P362, DOI 10.1007/s00535-017-1415-1
  50. Targher G, 2017, NAT REV NEPHROL, V13, P297, DOI 10.1038/nrneph.2017.16
  51. TOBIAS PS, 1995, J BIOL CHEM, V270, P10482, DOI 10.1074/jbc.270.18.10482
  52. Younossi ZM, 2016, HEPATOLOGY, V64, P73, DOI 10.1002/hep.28431
  53. Zhang X, 2018, PROTEIN CELL, V9, P164, DOI 10.1007/s13238-017-0436-0

Coleções
Artigos e Materiais de Revistas Científicas - FM/MGT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/35