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Title: | First Detection of DS-1-like G1P[8] Double-gene Reassortant Rotavirus Strains on The American Continent, Brazil, 2013 |
Authors: | LUCHS, Adriana; COSTA, Antonio Charlys da; CILLI, Audrey; KOMNINAKIS, Shirley Cavalcante Vasconcelos; CARMONA, Rita de Cassia Compagnoli; MORILLO, Simone Guadagnucci; SABINO, Ester Cerdeira; TIMENETSKY, Maria do Carmo Sampaio Tavares |
Citation: | SCIENTIFIC REPORTS, v.9, article ID 2210, 7p, 2019 |
Abstract: | Emergence of DS-1-like-G1P[8] rotavirus in Asia have been recently reported. We report for the first time the detection and the whole genome phylogenetic analysis of DS-1-like-G1P[8] strains in America. From 2013 to 2017, a total of 4226 fecal samples were screened for rotavirus by ELISA, PAGE, RT-PCR and sequencing. G1P[8] represented 3.7% (30/800) of all rotavirus-positive samples. DS-1-like-G1P[8] comprised 1.6% (13/800) detected exclusively in 2013, and Wa-like-G1P[8] comprised 2.1% (17/800) detected from 2013 to 2015. Whole genome sequencing confirmed the DS-1-like backbone I2-R2-C2-M2-A2-N2-T2-E2-H2. All genome segments of the Brazilian DS-1-like-G1P[8] strains clustered with those of Asian strains, and apart from African DS-1-like-G1P[8] strains. In addition, Brazilian DS-1-likeG1P[8] reassortants distantly clustered with DS-1-like backbone strains simultaneously circulating in the country, suggesting that the Brazilian DS-1-like-G1P[8] strains are likely imported from Asia. Two distinct NSP4 E2 genotype lineages were also identified, indicating the existence of a co-circulating pool of different DS-1-like G1P[8] strains. Surveillance systems must be developed to examine if RVA vaccines are still effective for the prevention against unusual DS-1-like-G1P[8] strains. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MIP Artigos e Materiais de Revistas Científicas - IMT Artigos e Materiais de Revistas Científicas - LIM/46 Artigos e Materiais de Revistas Científicas - ODS/03 |
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art_LUCHS_First_Detection_of_DS1like_G1P8_Doublegene_Reassortant_Rotavirus_2019.PDF | publishedVersion (English) | 2.73 MB | Adobe PDF | View/Open |
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