Multiplex-PCR for diagnosis of bacterial meningitis

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art_ALBUQUERQUE_MultiplexPCR_for_diagnosis_of_bacterial_meningitis_2019.PDF (513.72 KB)
Citações na Scopus
20
Tipo de produção
article
Data de publicação
2019
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
SPRINGER
Autores
ALBUQUERQUE, Renata Chaves
MORENO, Ana Carolina Ramos
RAGAZZI, Selma Lopes Betta
MARTINEZ, Marina Baquerizo
Citação
BRAZILIAN JOURNAL OF MICROBIOLOGY, v.50, n.2, p.435-443, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Considering the great lethality and sequels caused by meningitis, rapid diagnosis and prompt treatment initiation have a great impact on patient outcome. Here, we developed a multiplex-PCR for simultaneous detection of the four most prevalent bacterial pathogens directly in CSF samples. The multiplex-PCR was designed to detect the following genes: fbsA (Streptococcus agalactiae), lytA (Streptococcus pneumoniae), crtA (Neisseria meningitidis), p6 (Haemophilus influenzae), and 16S rRNA (any bacterial agent). The multiplex-PCR showed a DNA detection limit of 1pg/L. Among 447 CSF samples tested, 40 were multiplex-PCR positive, in which 27 and 13 had positive and negative bacterial culture, respectively. Our multiplex-PCR is fast, reliable, and easily implementable into a laboratory routine for bacterial meningitis confirmation, especially for patients who previously started antimicrobial therapy. Our molecular approach can substantially improve clinical diagnosis and epidemiological measures of meningitis disease burden.
Palavras-chave
Meningitis, Multiplex-PCR, Cerebrospinal fluid samples, Bacterial pathogens
URI
https://observatorio.fm.usp.br/handle/OPI/32021
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Coleções
Artigos e Materiais de Revistas Científicas - HU
Artigos e Materiais de Revistas Científicas - ODS/03