Pain inhibition through transplantation of fetal neuronal progenitors into the injured spinal cord in rats

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art_BATISTA_Pain_inhibition_through_transplantation_of_fetal_neuronal_progenitors_2019.PDF (3.95 MB)
Citações na Scopus
10
Tipo de produção
article
Data de publicação
2019
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
WOLTERS KLUWER MEDKNOW PUBLICATIONS
Autores
DALE, Camila S.
BRITTO, Luiz R.
MORGALLA, Matthias
Citação
NEURAL REGENERATION RESEARCH, v.14, n.11, p.2011-2019, 2019
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Neuropathic pain after spinal cord injury (SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy; nevertheless, the ideal cell type in terms of neurogenic potential and effectiveness against pain remains largely controversial. Here, we evaluated the ability of fetal neural stem cells (fNSC) to relieve chronic pain and, secondarily, their effects on motor recovery. Adult Wistar rats with traumatic SCI were treated, 10 days after injury, with intra-spinal injections of culture medium (sham) or fNSCs extracted from telencephalic vesicles (TV group) or the ventral medulla (VM group) of E/14 embryos. Sensory (von Frey filaments and hot plate) and motor (the Basso, Beattie, Bresnahan locomotor rating scale and inclined plane test) assessments were performed during 8 weeks. Thereafter, spinal cords were processed for immunofluorescence and transplanted cells were quantified by stereology. The results showed improvement of thermal hyperalgesia in the TV and VM groups at 4 and 5 weeks after transplantation, respectively. Moreover, mechanical allodynia improved in both the TV and VM groups at 8 weeks. No significant motor recovery was observed in the TV or VM groups compared with sham. Stereological analyses showed that similar to 70% of TV and VM cells differentiated into NeuN(+) neurons, with a high proportion of enkephalinergic and GABAergic cells in the TV group and enkephalinergic and serotoninergic cells in the VM group. Our study suggests that neuronal precursors from TV and VM, once implanted into the injured spinal cord, maturate into different neuronal subtypes, mainly GABAergic, serotoninergic, and enkephalinergic, and all subtypes alleviate pain, despite no significant motor recovery. The study was approved by the Animal Ethics Committee of the Medical School of the University of Sao Paulo (protocol number 033/14) on March 4, 2016.
Palavras-chave
spinal cord injuries, chronic pain, neural stem cells, cell transplantation, neuronal differentiation, GABAergic neuron, serotoninergic neuron, enkephalinergic neuron
URI
https://observatorio.fm.usp.br/handle/OPI/33132
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MOT
Artigos e Materiais de Revistas Científicas - FM/MCG
Artigos e Materiais de Revistas Científicas - FM/MNE
Artigos e Materiais de Revistas Científicas - HC/IPq
Artigos e Materiais de Revistas Científicas - HU
Artigos e Materiais de Revistas Científicas - LIM/26
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