C-4 and crassulacean acid metabolism within a single leaf: deciphering key components behind a rare photosynthetic adaptation

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Arquivos
art_FERRARI_C4_and_crassulacean_acid_metabolism_within_a_single_2020.PDF.pdf (3.2 MB)
Citações na Scopus
25
Tipo de produção
article
Data de publicação
2020
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
WILEY
Autores
FERRARI, Renata C.
BITTENCOURT, Priscila P.
RODRIGUES, Maria A.
MORENO-VILLENA, Jose J.
ALVES, Frederico R. R.
BOXALL, Susanna F.
DEVER, Louisa V.
DEMARCO, Diego
ANDRADE, Sonia C. S.
Citação
NEW PHYTOLOGIST, v.225, n.4, p.1699-1714, 2020
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Although biochemically related, C-4 and crassulacean acid metabolism (CAM) systems are expected to be incompatible. However, Portulaca species, including P. oleracea, operate C-4 and CAM within a single leaf, and the mechanisms behind this unique photosynthetic arrangement remain largely unknown. Here, we employed RNA-seq to identify candidate genes involved exclusively or shared by C-4 or CAM, and provided an in-depth characterization of their transcript abundance patterns during the drought-induced photosynthetic transitions in P. oleracea. Data revealed fewer candidate CAM-specific genes than those recruited to function in C-4. The putative CAM-specific genes were predominantly involved in night-time primary carboxylation reactions and malate movement across the tonoplast. Analysis of gene transcript-abundance regulation and photosynthetic physiology indicated that C-4 and CAM coexist within a single P. oleracea leaf under mild drought conditions. Developmental and environmental cues were shown to regulate CAM expression in stems, whereas the shift from C-4 to C-4-CAM hybrid photosynthesis in leaves was strictly under environmental control. Moreover, efficient starch turnover was identified as part of the metabolic adjustments required for CAM operation in both organs. These findings provide insights into C-4/CAM connectivity and compatibility, contributing to a deeper understanding of alternative ways to engineer CAM into C-4 crop species.
Palavras-chave
C-4, crassulacean acid metabolism, drought stress, facultative CAM, Portulaca oleracea, RNA-seq, transcriptome
URI
https://observatorio.fm.usp.br/handle/OPI/35697
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Coleções
Artigos e Materiais de Revistas Científicas - HC/IPq
Artigos e Materiais de Revistas Científicas - LIM/23