Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors

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art_CASOTTI_Molecular_detection_of_prepatent_Schistosoma_mansoni_infection_in_2020.PDF (416.29 KB)
Citações na Scopus
2
Tipo de produção
article
Data de publicação
2020
DOI
SciELO
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
INST MEDICINA TROPICAL SAO PAULO
Autores
TUAN, Roseli
Citação
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, v.62, article ID e17, 4p, 2020
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Approximately 240 million people worldwide are infected by Schistosoma. In Brazil, one of the main intermediate hosts of this parasite is Biomphalaria glabrata snails. The early detection of larval stages in intermediate hosts is an important challenge to public health. but it also represents an opportunity as a new alternative to indicate earlier natural infections before cercariae differentiation and emergence. In this context, we demonstrated that PCR amplification of a 28S gene fragment from the parasite does demonstrate S. mansoni infection in snails 14 days post infection. This conventional polymerase chain reaction amplified clear bands and was able to detect parasitic infection in the intermediate host B. glabrata under experimental conditions. However, we reinforce that this approach requires deeper investigations and further comparisons to confirm its specificity and sensitivity in earlier time points after miracidia infection. This approach has relevant potential as an effective molecular-based strategy for the monitoring of schistosomiasis transmission.
Palavras-chave
Schistosoma mansoni, Biomphalaria glabrata, Molecular diagnosis, Surveillance, Snail, Vector, Molecular diagnosis, Polymerase Chain Reaction
URI
https://observatorio.fm.usp.br/handle/OPI/35972
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - FM/MPR
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/03
Artigos e Materiais de Revistas Científicas - LIM/06
Artigos e Materiais de Revistas Científicas - LIM/07
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