Aerobic Exercise Training Exerts Beneficial Effects Upon Oxidative Metabolism and Non-Enzymatic Antioxidant Defense in the Liver of Leptin Deficiency Mice
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Citações na Scopus
12
Tipo de produção
article
Data de publicação
2020
Título da Revista
ISSN da Revista
Título do Volume
Editora
FRONTIERS MEDIA SA
Autores
LIMA, Talitta Ricarlly Lopes de Arruda
FERNANDES, Mariana Pinheiro
COGLIATI, Bruno
LAGRANHA, Claudia Jacques
Citação
FRONTIERS IN ENDOCRINOLOGY, v.11, article ID 588502, 9p, 2020
Resumo
Non-alcoholic fatty liver disease (NAFLD) is one of the most common forms of liver disease, which is associated with several etiological factors, including stress and dysfunction in oxidative metabolism. However, studies showed that aerobic exercise training (AET) can combat the oxidative stress (OS) and improves mitochondrial functionality in the NAFLD. To test the hypothesis that AET improves oxidative metabolism and antioxidant defense in the liver of ob/ob mice. Male ob/ob mice with eight weeks old were separated into two groups: the sedentary group (S), n=7, and the trained group (T), n=7. The T mice were submitted to an 8-week protocol of AET at 60% of the maximum velocity achieved in the running capacity test. Before AET, no difference was observed in running test between the groups (S=10.4 +/- 0.7 min vs. T= 13 +/- 0.47 min). However, after AET, the running capacity was increased in the T group (12.8 +/- 0.87 min) compared to the S group (7.2 +/- 0.63 min). In skeletal muscle, the T group (26.91 +/- 1.12 U/mg of protein) showed higher citrate synthase activity compared with the S group (19.28 +/- 0.88 U/mg of protein) (p =0.006). In the analysis of BW evolution, significant reductions were seen in the T group as of the fourth week when compared to the S group. In addition, food intake was not significant different between the groups. Significant increases were observed in the activity of enzymes citrate synthase (p=0.004) and beta-HAD (p=0.01) as well as in PGC-1 alpha gene expression (p=0.002) in the liver of T group. The levels of TBARs and carbonyls, as well as SOD, CAT and GST were not different between the groups. However, in the nonenzymatic antioxidant system, we found that the T group had higher sulfhydryl (p = 0.02), GSH (p=0.001) and GSH/GSSG (p=0.02) activity. In conclusion, the AET improved body weight evolution and the aerobic capacity, increased the response of oxidative metabolism markers in the liver such as PGC-1 alpha gene expression and citrate synthase and beta-HAD enzyme activities in ob/ob mice. In addition, AET improved the non-enzymatic antioxidant defense and did not change the enzymatic defense.
Palavras-chave
liver disease, oxidative metabolism, antioxidant defense, physical exercise, leptin deficiency
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