Low-dose chlorine exposure impairs lung function, inflammation and oxidative stress in mice

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art_GENARO_Lowdose_chlorine_exposure_impairs_lung_function_inflammation_and_2021.PDF (4.8 MB)
Citações na Scopus
12
Tipo de produção
article
Data de publicação
2021
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
PERGAMON-ELSEVIER SCIENCE LTD
Autores
GENARO, Isabella Santos de
ALMEIDA, Francine Maria de
KUNZLER, Deborah De Camargo Hizume
TRIPODE, Bruna Gabryela Busoletto
KURDEJAK, Adriana
CORDEIRO, Bruna Nakamura
PANDOLPHO, Renata
BRUGGEMANN, Thayse Regina
Citação
LIFE SCIENCES, v.267, article ID 118912, 11p, 2021
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Aim: To explore the different consequences of acute and chronic exposure to chlorine gas (Cl-2) on the functional and histological parameters of health mice. Main methods: Firstly, male BALB/c mice were acute exposed to 3.3 or 33.3 or 70.5 mg/m(3) Cl-2. We analyzed the lung function, the inflammatory cells in the bronchoalveolar lavage, cell influx in the peribrochoalveolar space and mucus production. In a second phase, mice were chronic exposed to 70.5 mg/m(3) Cl-2. Besides the first phase analyses, we also evaluated the epithelial cells thickness, collagen deposition in the airways, immunohisto-chemistry stain for IL-1 beta, iNOS, IL-17 and ROCK-2 and the levels of IL-5, IL-13, IL-17, IL-1 beta and TNF-alpha in lung homogenate. Key findings: Acute exposure to chlorine impaired the lung function, increased the number of inflammatory cells in the BALF and in the airways, also increased the mucus production. Furthermore, when chlorine was exposed chronically, increased the airway remodeling with collagen deposition and epithelial cells thickness, positive cells for IL-1 beta, iNOS, IL-17 in the airways and in the alveolar walls and ROCK-2 in the alveolar walls, lung inflammation with increased levels of IL-5, IL-13, IL-1 beta and TNF-alpha in the lung homogenate, and also, induced the acid mucus production by the nasal epithelium. Significance: Acute and chronic exposure to low dose of chlorine gas worsens lung function, induces oxidative stress activation and mucus production and contributes to augmenting inflammation in health mice.
Palavras-chave
Sodium hypochlorite, Lung function, Lung inflammation, Pulmonary remodeling, Oxidative stress
URI
https://observatorio.fm.usp.br/handle/OPI/39709
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Coleções
Artigos e Materiais de Revistas Científicas - FM/MCM
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/05
Artigos e Materiais de Revistas Científicas - LIM/20
Artigos e Materiais de Revistas Científicas - ODS/03