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https://observatorio.fm.usp.br/handle/OPI/4057
Title: | A Fast and Cost-Effective Method for Identifying a Polymorphism of Interleukin 28B Related to Hepatitis C |
Authors: | FERREIRA, Camila da Silva; ABREU, Rodrigo Martins; SILVA, Marlone Cunha da; FERREIRA, Aline Siqueira; NASSER, Paulo Dominguez; CARRILHO, Flair Jose; ONO, Suzane Kioko |
Citation: | PLOS ONE, v.8, n.10, article ID e78142, 4p, 2013 |
Abstract: | Approximately 170 million people are chronic carriers of hepatitis C virus (HCV). Patients with chronic hepatitis C are currently treated with pegylated interferon and ribavirin (PEG-IFN/RBV). A genome-wide association with PEG-IFN/RBV treatment response and a single nucleotide polymorphism (rs12979860) has been identified near the interleukin 28B gene that encodes interferon-lambda-3. In this paper, we describe an innovative, fast, and low-cost multiplex polymerase chain reaction with confronting two-pair primers that detects the rs12979860 polymorphism. The assay is internally controlled and does not require the use of restriction endonucleases or special equipment. Moreover, the assay decreases costs, being about 40% cheaper than direct sequencing methods. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MGT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/07 Artigos e Materiais de Revistas Científicas - ODS/03 |
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File | Description | Size | Format | |
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art_FERREIRA_A_Fast_and_Cost_Effective_Method_for_Identifying_2013.PDF | publishedVersion (English) | 241.28 kB | Adobe PDF | View/Open |
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