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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | TEIXEIRA, Leandro Emidio | - |
dc.contributor.author | KANUNFRE, Kelly Aparecida | - |
dc.contributor.author | SHIMOKAWA, Paulo Tadashi | - |
dc.contributor.author | TARGA, Lilia Spaleta | - |
dc.contributor.author | RODRIGUES, Jonatas Cristian | - |
dc.contributor.author | DOMINGUES, Wilson | - |
dc.contributor.author | YAMAMOTO, Lidia | - |
dc.contributor.author | OKAY, Thelma Suely | - |
dc.date.accessioned | 2014-01-28T22:25:57Z | - |
dc.date.available | 2014-01-28T22:25:57Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, v.46, n.5, p.584-588, 2013 | - |
dc.identifier.issn | 0037-8682 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/4178 | - |
dc.description.abstract | Introduction: Toxoplasmosis may be life-threatening in fetuses and in immune-deficient patients. Conventional laboratory diagnosis of toxoplasmosis is based on the presence of IgM and IgG anti-Toxoplasma gondii antibodies; however, molecular techniques have emerged as alternative tools due to their increased sensitivity. The aim of this study was to compare the performance of 4 PCR-based methods for the laboratory diagnosis of toxoplasmosis. One hundred pregnant women who seroconverted during pregnancy were included in the study. The definition of cases was based on a 12-month follow-up of the infants. Methods: Amniotic fluid samples were submitted to DNA extraction and amplification by the following 4 Toxoplasma techniques performed with parasite B1 gene primers: conventional PCR, nested-PCR, multiplex-nested-PCR, and real-time PCR. Seven parameters were analyzed, sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (PLR), negative likelihood ratio (NLR) and efficiency (Ef). Results: Fifty-nine of the 100 infants had toxoplasmosis; 42 (71.2%) had IgM antibodies at birth but were asymptomatic, and the remaining 17 cases had non-detectable IgM antibodies but high IgG antibody titers that were associated with retinochoroiditis in 8 (13.5%) cases, abnormal cranial ultrasound in 5 (8.5%) cases, and signs/symptoms suggestive of infection in 4 (6.8%) cases. The conventional PCR assay detected 50 cases (9 false-negatives), nested-PCR detected 58 cases (1 false-negative and 4 false-positives), multiplex-nested-PCR detected 57 cases (2 false-negatives), and real-time-PCR detected 58 cases (1 false-negative). Conclusions: The real-time PCR assay was the best-performing technique based on the parameters of Se (98.3%), Sp (100%), PPV (100%), NPV (97.6%), PLR (co), NLR (0.017), and Ef (99%). | - |
dc.description.sponsorship | FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [2010/15022-1] | - |
dc.description.sponsorship | CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [2011-0/471479] | - |
dc.language.iso | eng | - |
dc.publisher | SOC BRASILEIRA MEDICINA TROPICAL | - |
dc.relation.ispartof | Revista da Sociedade Brasileira de Medicina Tropical | - |
dc.rights | openAccess | - |
dc.subject | Congenital toxoplasmosis | - |
dc.subject | Congenital infection | - |
dc.subject | Molecular diagnosis | - |
dc.subject | PCR | - |
dc.subject | Quantitative PCR | - |
dc.subject.other | real-time pcr | - |
dc.subject.other | gondii | - |
dc.subject.other | infection | - |
dc.subject.other | af146527 | - |
dc.subject.other | children | - |
dc.subject.other | repeat | - |
dc.title | The performance of four molecular methods for the laboratory diagnosis of congenital toxoplasmosis in amniotic fluid samples | - |
dc.type | article | - |
dc.rights.holder | Copyright SOC BRASILEIRA MEDICINA TROPICAL | - |
dc.identifier.doi | 10.1590/0037-8682-0095-2013 | - |
dc.identifier.pmid | 24409481 | - |
dc.subject.wos | Tropical Medicine | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | TEIXEIRA, Leandro Emidio:Univ Sao Paulo, Inst Med Trop Sao Paulo, Lab Soroepidemiol & Imunobiol, Sao Paulo, Brazil | - |
hcfmusp.author.external | DOMINGUES, Wilson:Univ Sao Paulo, Inst Med Trop Sao Paulo, Lab Soroepidemiol & Imunobiol, Sao Paulo, Brazil | - |
hcfmusp.description.beginpage | 584 | - |
hcfmusp.description.endpage | 588 | - |
hcfmusp.description.issue | 5 | - |
hcfmusp.description.volume | 46 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | 2-s2.0-84888156919 | - |
hcfmusp.origem.id | WOS:000327672500009 | - |
hcfmusp.origem.id | SCIELO:S0037-86822013000500584 | - |
hcfmusp.publisher.city | BRASILIA | - |
hcfmusp.publisher.country | BRAZIL | - |
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dc.description.index | MEDLINE | - |
hcfmusp.remissive.sponsorship | CNPq | - |
hcfmusp.remissive.sponsorship | FAPESP | - |
hcfmusp.citation.scopus | 37 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPE Artigos e Materiais de Revistas Científicas - HC/ICr Artigos e Materiais de Revistas Científicas - IMT Artigos e Materiais de Revistas Científicas - LIM/36 Artigos e Materiais de Revistas Científicas - LIM/48 |
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