The Trypanosoma cruzi Antigen and Epitope Atlas: antibody specificities in Chagas disease patients across the Americas

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art_RICCI_The_Trypanosoma_cruzi_Antigen_and_Epitope_Atlas_antibody_2023.PDF (2.81 MB)
Citações na Scopus
6
Tipo de produção
article
Data de publicação
2023
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
NATURE PORTFOLIO
Autores
RICCI, Alejandro D.
BRACCO, Leonel
SALAS-SARDUY, Emir
RAMSEY, Janine M.
NOLAN, Melissa S.
LYNN, M. Katie
ALTCHEH, Jaime
BALLERING, Griselda E.
TORRICO, Faustino
Citação
NATURE COMMUNICATIONS, v.14, n.1, 2023
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
During an infection the immune system produces pathogen-specific antibodies. These antibody repertoires become specific to the history of infections and represent a rich source of diagnostic markers. However, the specificities of these antibodies are mostly unknown. Here, using high-density peptide arrays we examined the human antibody repertoires of Chagas disease patients. Chagas disease is a neglected disease caused by Trypanosoma cruzi, a protozoan parasite that evades immune mediated elimination and mounts long-lasting chronic infections. We describe a proteome-wide search for antigens, characterised their linear epitopes, and show their reactivity on 71 individuals from diverse human populations. Using single-residue mutagenesis we revealed the core functional residues for 232 of these epitopes. Finally, we show the diagnostic performance of identified antigens on challenging samples. These datasets enable the study of the Chagas antibody repertoire at an unprecedented depth and granularity, while also providing a rich source of serological biomarkers. This work reveals the diversity and extent of human antibody specificities in Chagas disease and provides a wealth of well-defined antigenic markers for diagnosis and development of serological applications for this neglected infectious disease.
Palavras-chave
URI
https://observatorio.fm.usp.br/handle/OPI/54901
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Coleções
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/49