Characterization of Torquetenovirus in amniotic fluid at the time of <i>in utero</i> fetal surgery: correlation with early premature delivery and respiratory distress
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Citações na Scopus
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Tipo de produção
article
Data de publicação
2023
Título da Revista
ISSN da Revista
Título do Volume
Editora
FRONTIERS MEDIA SA
Citação
FRONTIERS IN MEDICINE, v.10, article ID 1161091, 8p, 2023
Resumo
Torquetenovirus (TTV) is a commensal virus present in many healthy individuals. Although considered to be non-pathogenic, its presence and titer have been shown to be indicative of altered immune status in individuals with chronic infections or following allogeneic transplantations. We evaluated if TTV was present in amniotic fluid (AF) at the time of in utero surgery to correct a fetal neurological defect, and whether its detection was predictive of adverse post-surgical parameters. AF was collected from 27 women by needle aspiration prior to a uterine incision. TTV titer in the AF was measured by isolation of viral DNA followed by gene amplification and analysis. The TTV genomes were further characterized and sequenced by metagenomics. Pregnancy outcome parameters were subsequently obtained by chart review. Three of the AFs (11.1%) were positive for TTV at 3.36, 4.16, and 4.19 log(10) copies/mL. Analysis of their genomes revealed DNA sequences similar to previously identified TTV isolates. Mean gestational age at delivery was >2 weeks earlier (32.5 vs. 34.6 weeks) and the prevalence of respiratory distress was greater (100% vs. 20.8%) in the TTV-positive pregnancies. TTV detection in AF prior to intrauterine surgery may indicate elevated post-surgical risk for earlier delivery and newborn respiratory distress.
Palavras-chave
amniotic fluid, fetal surgery, preterm birth, respiratory distress, spina bifida, Torquetenovirus
Referências
- Adzick NS, 2011, NEW ENGL J MED, V364, P993, DOI 10.1056/NEJMoa1014379
- Biagini P, 2009, CURR TOP MICROBIOL, V331, P21
- Biomatters, 2023, GEN VERS 2023 0
- Bustin SA, 2009, CLIN CHEM, V55, P611, DOI 10.1373/clinchem.2008.112797
- Bzhalava D, 2012, VIROLOGY, V432, P427, DOI 10.1016/j.virol.2012.06.022
- Cavalheiro S, 2020, J NEUROSURG-PEDIATR, V26, P605, DOI 10.3171/2020.3.PEDS19613
- Chan PKS, 2001, CLIN INFECT DIS, V32, P1376, DOI 10.1086/319983
- da Costa AC, 2017, EMERG INFECT DIS, V23, P1742, DOI 10.3201/eid2310.170307
- Debiaggi M, 2000, CLIN MICROBIOL INFEC, V6, P246, DOI 10.1046/j.1469-0691.2000.00069.x
- Deng XT, 2015, NUCLEIC ACIDS RES, V43, DOI 10.1093/nar/gkv002
- Desai SM, 1999, J INFECT DIS, V179, P1242, DOI 10.1086/314735
- Desingu PA., 2022, FRONT VIROL, V2, P821298, DOI [10.3389/fviro.2022.821298, DOI 10.3389/FVIRO.2022.821298]
- Emery SL, 2004, EMERG INFECT DIS, V10, P311, DOI 10.3201/eid1002.030759
- Fernández-Ruiz M, 2020, AM J TRANSPLANT, V20, P1963, DOI 10.1111/ajt.15872
- Hutchins GM, 1996, PEDIATR PATHOL LAB M, V16, P701, DOI 10.1080/107710496175327
- Kakkola L, 2008, VIROLOGY, V382, P182, DOI 10.1016/j.virol.2008.09.012
- Katoh K, 2002, NUCLEIC ACIDS RES, V30, P3059, DOI 10.1093/nar/gkf436
- Kumar S, 2018, MOL BIOL EVOL, V35, P1547, DOI 10.1093/molbev/msy096
- Leijonhufvud G, 2022, J MED VIROL, V94, P634, DOI 10.1002/jmv.27409
- Maggi F, 2001, J VIROL, V75, P11999, DOI 10.1128/JVI.75.24.11999-12004.2001
- Matsubara H, 2001, HEPATOL RES, V21, P280, DOI 10.1016/S1386-6346(01)00115-2
- Moron AF, 2018, BJOG-INT J OBSTET GY, V125, P1280, DOI 10.1111/1471-0528.15312
- Moron AF, 2018, BJOG-INT J OBSTET GY, V125, P1288, DOI 10.1111/1471-0528.15298
- Mrzljak Anna, 2020, World J Transplant, V10, P291, DOI 10.5500/wjt.v10.i11.291
- Okonechnikov K, 2012, BIOINFORMATICS, V28, P1166, DOI 10.1093/bioinformatics/bts091
- Posada D, 2008, MOL BIOL EVOL, V25, P1253, DOI 10.1093/molbev/msn083
- Price MN, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0009490
- Ravi KS, 2021, J FAM MED PRIM CARE, V10, P4383, DOI 10.4103/jfmpc.jfmpc_904_21
- Ross RS, 1999, J CLIN VIROL, V13, P181, DOI 10.1016/S1386-6532(99)00015-3
- Tcherepanov V, 2006, BMC GENOMICS, V7, DOI 10.1186/1471-2164-7-150
- Tourinho RS., 2015, J GENET GENOME RES, V2, P1, DOI [10.23937/2378-3648/1410013, DOI 10.23937/2378-3648/1410013]
- Tozetto-Mendoza TR, 2020, REPROD SCI, V27, P2075, DOI 10.1007/s43032-020-00227-1
- Underwood Mark A, 2005, J Perinatol, V25, P341, DOI 10.1038/sj.jp.7211290
Coleções
Artigos e Materiais de Revistas Científicas - FM/MIP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/46
Artigos e Materiais de Revistas Científicas - LIM/52
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - HC/InCor
Artigos e Materiais de Revistas Científicas - IMT
Artigos e Materiais de Revistas Científicas - LIM/46
Artigos e Materiais de Revistas Científicas - LIM/52