Analysis of Hepatitis C Virus Intrahost Diversity across the Coding Region by Ultradeep Pyrosequencing
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Citações na Scopus
41
Tipo de produção
article
Data de publicação
2012
Título da Revista
ISSN da Revista
Título do Volume
Editora
AMER SOC MICROBIOLOGY
Autores
LAUCK, Michael
BECKER, Ericka A.
BHATTACHARYA, Dipankar
STRIKER, Rob
HUGHES, Austin L.
O'CONNOR, David H.
Citação
JOURNAL OF VIROLOGY, v.86, n.7, p.3952-3960, 2012
Resumo
Hepatitis C virus (HCV) is the leading cause of liver disease worldwide. In this study, we analyzed four treatment-naive patients infected with subtype 1a and performed Roche/454 pyrosequencing across the coding region. We report the presence of low-level drug resistance mutations that would most likely have been missed using conventional sequencing methods. The approach described here is broadly applicable to studies of viral diversity and could help to improve the efficacy of direct-acting antiviral agents (DAA) in the treatment of HCV-infected patients.
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Referências
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