Best Practice for Quantifying the Microscopic Structure of Mouse Placenta: The Stereological Approach
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Data de publicação
2014
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Veras, Mariana M.; Costa, Natália S.X.; Mayhew, Terry. Best Practice for Quantifying the Microscopic Structure of Mouse Placenta: The Stereological Approach. In: Croy, Anne; Yamada, Aureo T.; DeMayo, Francesco J.; Adamson, S. Lee (eds). The Guide to Investigation of Mouse Pregnancy. SAN DIEGO: ELSEVIER, 2014. p.545-556.
Resumo
Stereology provides a set of efficient and unbiased or minimally biased tools for quantifying functionally relevant aspects of the three-dimensional morphology of the placenta. The quantities include volumes, surface areas, lengths, and numbers and can be estimated for different levels of structural organization, from whole organ to cell and molecule. By paying careful attention to how the placenta is sampled for microscopic examination, stereological tools can be used to shed light on relevant processes, such as placental growth and development, oxygen transport, fetoplacental angiogenesis, and trophoblast turnover. Here, we review stereological best practices as applied to the mouse placenta during normal pregnancy and after experimental manipulation.
Palavras-chave
Fetal capillaries, Intervascular membrane, Junctional zone, Labyrinth, Maternal blood space, Microscopy, Stereology, Trophoblast