New insights about cross-reactive epitopes of six trypanosomatid genera revealed that Crithidia and Leptomonas have antigenic similarity to L. (L.) chagasi

Imagem de Miniatura
Arquivos
art_FERREIRA_New_insights_about_cross_reactive_epitopes_of_six_2014.PDF (988.09 KB)
Citações na Scopus
12
Tipo de produção
article
Data de publicação
2014
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
ELSEVIER SCIENCE BV
Autores
TEIXEIRA, Marta M. G.
BARBIERI, C. L.
LINDOSO, Jose Angelo
Citação
ACTA TROPICA, v.131, p.41-46, 2014
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
We investigated whether ELISA using crude antigens from insect and plant trypanosomatids, which are non-pathogenic and easily cultivated in large scale, has the same positivity data as Leishmania (Leishmania) chagasi, the etiological agent of human visceral leishmaniasis (VL) or canine leishmaniasis (CanL), or as Trypanosoma cruzi, the etiological agent of Chagas disease (CD). The antigens from Crithidia fasciculata, Crithidia luciliae, and Leptomonas seymouri showed 100% cross-reactivity with VL and CanL samples, with no statistically titers differences from L. (L.) chagasi, however, 34% (17/50) of VL samples revealed higher titers using the insect trypanosomatids than the homologous antigen. On the other hand, antigens from Strigomonas culicis, Angomonas deanei, and Phytomonas serpens showed low cross-reactivity with VL and CanL samples. The sera from patients with American tegumentary leishmaniasis showed low levels of cross-reactivity with all trypanosomatids investigated, even with L. (L) chagasi, without titers dissimilarity among them. These parasites were also worthless as antigen source for detection of CD cases, which required homologous antigens to reach 100% positivity. This study showed, by ELISA, that crude extract of Crithidia and Leptomonas have epitopes similar to L. (L.) chagasi, which supports the idea of using them as antigens source for the serodiagnosis of visceral leishmaniasis.
Palavras-chave
L. (L.) chagasi, Trypanosoma cruzi, Crithidia, Leptomonas, Trypanosomatids, Cross-reactivity
URI
https://observatorio.fm.usp.br/handle/OPI/7700
Referências
  1. AARDEN LA, 1975, ANN NY ACAD SCI, V254, P505, DOI 10.1111/j.1749-6632.1975.tb29197.x
  2. Borghesan TC, 2013, PROTIST, V164, P129, DOI 10.1016/j.protis.2012.06.001
  3. Bregano JW, 2003, FEMS IMMUNOL MED MIC, V39, P257, DOI 10.1016/S0928-8244(03)00256-6
  4. Camargo EP, 1999, ADV PARASIT, V42, P29, DOI 10.1016/S0065-308X(08)60148-7
  5. Chicharro C, 2003, ANN TROP MED PARASIT, V97, P75, DOI 10.1179/000349803225002552
  6. de Souza Dias Suzana, 2005, Am J Trop Med Hyg, V72, P126
  7. Depledge DP, 2010, PLOS NEGLECT TROP D, V4, DOI 10.1371/journal.pntd.0000829
  8. Desquesnes M, 2007, INFECT GENET EVOL, V7, P457, DOI 10.1016/j.meegid.2006.01.007
  9. ETGES R, 1992, ACTA TROP, V50, P205, DOI 10.1016/0001-706X(92)90077-B
  10. Ghosh S, 2012, J CLIN MICROBIOL, V50, P2774, DOI 10.1128/JCM.00966-12
  11. Goncalves CCM, 2002, AM J TROP MED HYG, V66, P91
  12. Graca-de Souza VK, 2010, BIOL RES, V43, P233, DOI /S0716-97602010000200011
  13. Griemberg G, 2006, MEDICINA-BUENOS AIRE, V66, P3
  14. HEDGE EC, 1978, T ROY SOC TROP MED H, V72, P445, DOI 10.1016/0035-9203(78)90154-2
  15. HOWARD MK, 1991, T ROY SOC TROP MED H, V85, P477, DOI 10.1016/0035-9203(91)90226-O
  16. Jackson AP, 2006, BMC GENOMICS, V7, DOI 10.1186/1471-2164-7-261
  17. Jackson AP, 2010, MOL BIOL EVOL, V27, P33, DOI 10.1093/molbev/msp214
  18. Jimenez MI, 1996, LANCET, V347, P264, DOI 10.1016/S0140-6736(96)90441-9
  19. Jirku M, 2012, J EUKARYOT MICROBIOL, V59, P537, DOI 10.1111/j.1550-7408.2012.00636.x
  20. LOPES JD, 1981, AM J TROP MED HYG, V30, P1183
  21. López-Brea M, 1979, Rev Sanid Hig Publica (Madr), V53, P1109
  22. Martinkovic F, 2006, PARASITOL RES, V98, P378, DOI 10.1007/s00436-005-0072-0
  23. Merzlyak E, 2001, J EUKARYOT MICROBIOL, V48, P161, DOI 10.1111/j.1550-7408.2001.tb00298.x
  24. Monteon VM, 1997, J CLIN MICROBIOL, V35, P3316
  25. Moreira MAB, 2007, VET PARASITOL, V145, P245, DOI 10.1016/j.vetpar.2006.12.012
  26. Morio F, 2008, J CLIN MICROBIOL, V46, P3845, DOI 10.1128/JCM.01098-08
  27. Pereira FM, 2010, PROTIST, V161, P589, DOI 10.1016/j.protis.2010.02.001
  28. Pinedo-Cancino V, 2013, AM J TROP MED HYG, V88, P559, DOI 10.4269/ajtmh.12-0587
  29. Pinge-Filho P, 2005, IMMUNOL LETT, V96, P283, DOI 10.1016/j.imlet.2004.09.010
  30. Rafati S, 2006, CLIN VACCINE IMMUNOL, V13, P1104, DOI 10.1128/CVI.00188-06
  31. Santos ALS, 2007, MICROBES INFECT, V9, P915, DOI 10.1016/j.micinf.2007.03.018
  32. Santos R.R., 1975, REV GOIANA MED, V21, P23
  33. Singh N, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0055738
  34. SOUZA MDC, 1974, J PROTOZOOL, V21, P579, DOI 10.1111/j.1550-7408.1974.tb03705.x
  35. Stuart K, 2008, J CLIN INVEST, V118, P1301, DOI 10.1172/JCI33945
  36. Teixeira MMG, 2011, PROTIST, V162, P503, DOI 10.1016/j.protis.2011.01.001
  37. Teixeira M.M.G., 2001, FEMS MICROBIOL LETT, V176, P241
  38. Umezawa ES, 2009, ACTA TROP, V111, P15, DOI 10.1016/j.actatropica.2009.01.006
  39. Umezawa ES, 1996, J CLIN MICROBIOL, V34, P2143
  40. Yao CQ, 2010, INFECT IMMUN, V78, P22, DOI 10.1128/IAI.00776-09

Coleções
Artigos e Materiais de Revistas Científicas - FM/MPT
Artigos e Materiais de Revistas Científicas - FM/MPR
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/36
Artigos e Materiais de Revistas Científicas - LIM/49
Artigos e Materiais de Revistas Científicas - LIM/50