A Hyperoxic Lung Injury Model in Premature Rabbits: The Influence of Different Gestational Ages and Oxygen Concentrations

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art_MANZANO_A_Hyperoxic_Lung_Injury_Model_in_Premature_Rabbits_2014.PDF (686.77 KB)
Citações na Scopus
13
Tipo de produção
article
Data de publicação
2014
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
PUBLIC LIBRARY SCIENCE
Autores
MANZANO, Roberta Munhoz
MASCARETTI, Renata Suman
CARRER, Valeria
HADDAD, Luciana Branco
FERNANDES, Aline Rabelo
REYES, Ana M. A.
Citação
PLOS ONE, v.9, n.4, article ID e95844, 8p, 2014
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
Background: Many animal models have been developed to study bronchopulmonary dysplasia (BPD). The preterm rabbit is a low-cost, easy-to-handle model, but it has a high mortality rate in response to the high oxygen concentrations used to induce lung injury. The aim of this study was to compare the mortality rates of two models of hyperoxia-induced lung injury in preterm rabbits. Methods: Pregnant New Zealand white rabbits were subjected to caesarean section on gestational day 28 or 29 (full term = 31 days). The premature rabbits in the 28-day gestation group were exposed to room air or FiO(2) >= 95%, and the rabbits in the 29-day gestation group were exposed to room air or FiO(2) = 80% for 11 days. The mean linear intercept (Lm), internal surface area ( ISA), number of alveoli, septal thickness and proportion of elastic and collagen fibers were quantified. Results: The survival rates in the 29-day groups were improved compared with the 28-day groups. Hyperoxia impaired the normal development of the lung, as demonstrated by an increase in the Lm, the septal thickness and the proportion of elastic fibers. Hyperoxia also decreased the ISA, the number of alveoli and the proportion of collagen fibers in the 28-day oxygen-exposed group compared with the control 28-day group. A reduced number of alveoli was found in the 29-day oxygen exposed animals compared with the control 29-day group. Conclusions: The 29-day preterm rabbits had a reduced mortality rate compared with the 28-day preterm rabbits and maintained a reduction in the alveoli number, which is comparable to BPD in humans.
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URI
https://observatorio.fm.usp.br/handle/OPI/7719
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Coleções
Artigos e Materiais de Revistas Científicas - FM/Outros
Artigos e Materiais de Revistas Científicas - HC/ICr