Binding Affinity, Specificity and Comparative Biodistribution of the Parental Murine Monoclonal Antibody MX35 (Anti-NaPi2b) and Its Humanized Version Rebmab200

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art_LINDEGREN_Binding_Affinity_Specificity_and_Comparative_Biodistribution_of_the_2015.PDF (2.66 MB)
Citações na Scopus
13
Tipo de produção
article
Data de publicação
2015
DOI
Scopus
Web of Science
PubMed
Título da Revista
ISSN da Revista
Título do Volume
Editora
PUBLIC LIBRARY SCIENCE
Autores
LINDEGREN, Sture
BACK, Tom
HORTA, Bruno Brasil
MORO, Ana Maria
OKAMOTO, Oswaldo Keith
JACOBSSON, Lars
CEDERKRANTZ, Elin
Citação
PLOS ONE, v.10, n.5, article ID e0126298, 16p, 2015
Projetos de Pesquisa
Unidades Organizacionais
Fascículo
Resumo
The aim of this preclinical study was to evaluate the characteristics of the monoclonal antibody Rebmab200, which is a humanized version of the ovarian-specific murine antibody MX35. This investigation contributes to the foundation for future clinical alpha-radioimmunotherapy of minimal residual ovarian cancer with At-211-Rebmab200. Here, the biodistribution of 211At-Rebmab200 was evaluated, as was the utility of Tc-99m-Rebmab200 for bioimaging. Rebmab200 was directly compared with its murine counterpart MX35 in terms of its in-vitro capacity for binding the immobilized NaPi2B epitope and live cells; we also assessed its biodistribution in nude mice carrying subcutaneous OVCAR-3 tumors. Tumor antigen and cell binding were similar between Rebmab200 and murine MX35, as was biodistribution, including normal tissue uptake and in-vivo tumor binding. We also demonstrated that Tc-99m-Rebmab200 can be used for single-photon emission computed tomography of subcutaneous ovarian carcinomas in tumor-bearing mice. Taken together, our data support the further development of Rebmab200 for radioimmunotherapy and diagnostics.
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URI
https://observatorio.fm.usp.br/handle/OPI/9748
Referências
  1. Andersson H, 2009, J NUCL MED, V50, P1153, DOI 10.2967/jnumed.109.062604
  2. Andersson H, 2000, ACTA ONCOL, V39, P741
  3. Andersson H, 2000, ANTICANCER RES, V20, P459
  4. Elgqvist J, 2006, INT J RADIAT ONCOL, V66, P1228, DOI 10.1016/j.ijrobp.2006.07.003
  5. Elgqvist J, 2005, J NUCL MED, V46, P464
  6. Elgqvist J, 2010, J ONCOL, V2010, DOI [10.1155/2010/394913, DOI 10.1155/2010/394913]
  7. Elgqvist J, 2005, J NUCL MED, V46, P1907
  8. Flook AM, 2013, MOL PHARMACEUT, V10, P3417, DOI 10.1021/mp400248f
  9. Gainkam LOT, 2008, J NUCL MED, V49, P788, DOI 10.2967/jnumed.107.048538
  10. Goldenberg DM, 2012, THERANOSTICS, V2, P523, DOI 10.7150/thno.3582
  11. HAMILTON JG, 1950, J CLIN INVEST, V29, P820
  12. KUUSREICHEL K, 1994, CLIN DIAGN LAB IMMUN, V1, P365
  13. Leinster DA, 2012, J PATHOL, V227, P136, DOI 10.1002/path.4002
  14. Lindegren S, 2001, APPL RADIAT ISOTOPES, V55, P157, DOI 10.1016/S0969-8043(01)00044-6
  15. Lindegren S, 2008, J NUCL MED, V49, P1537, DOI 10.2967/jnumed.107.049833
  16. LINDMO T, 1984, J IMMUNOL METHODS, V72, P77, DOI 10.1016/0022-1759(84)90435-6
  17. dos Santos ML, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0070332
  18. MAHER VE, 1992, J IMMUNOTHER, V11, P56, DOI 10.1097/00002371-199201000-00007
  19. Malviya G, 2012, MOL IMAGING BIOL, V14, P637, DOI 10.1007/s11307-011-0527-x
  20. MATTES MJ, 1987, CANCER RES, V47, P6741
  21. Mirick GR, 2004, Q J NUCL MED MOL IM, V48, P251
  22. Vaneycken I, 2010, J NUCL MED, V51, P1099, DOI 10.2967/jnumed.109.069823
  23. VISSER GWM, 1981, INT J APPL RADIAT IS, V32, P905, DOI 10.1016/0020-708X(81)90077-6
  24. Wu AM, 2014, METHODS, V65, P139, DOI 10.1016/j.ymeth.2013.09.015
  25. Yin Beatrice W T, 2008, Cancer Immun, V8, P3
  26. Zalutsky MR, 2005, J NUCL MED, V46, p151S
  27. Zalutsky MR, 2000, CURR PHARM DESIGN, V6, P1433, DOI 10.2174/1381612003399275
  28. Zalutsky MR, 2008, J NUCL MED, V49, P30, DOI 10.2967/jnumed.107.046938
  29. ZALUTSKY MR, 1987, APPL RADIAT ISOTOPES, V38, P1051

Coleções
Artigos e Materiais de Revistas Científicas - FM/MDR
Artigos e Materiais de Revistas Científicas - HC/ICESP
Artigos e Materiais de Revistas Científicas - HC/ICHC
Artigos e Materiais de Revistas Científicas - LIM/24
Artigos e Materiais de Revistas Científicas - LIM/43
Artigos e Materiais de Revistas Científicas - ODS/03