Western blotting method (TESAcruzi) as a supplemental test for confirming the presence of anti-Trypanosoma cruzi antibodies in finger prick blood samples from children aged 0-5 years in Brazil

dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.authorFRADE, Amanda Farage
dc.contributor.authorLUQUETTI, Alejandro O.
dc.contributor.authorPRATA, Aluisio
dc.contributor.authorFERREIRA, Antonio Walter
dc.date.accessioned2017-11-27T16:28:17Z
dc.date.available2017-11-27T16:28:17Z
dc.date.issued2011
dc.description.abstractSome Latin American countries have plans for total control and/or eradication of Chagas disease by the main vector (Triatoma infestans) and by blood transfusion. To achieve this, patients with Chagas disease must be identified. A Western blotting test, TESAcruzi, is described as a supplemental test for diagnosis of Chagas disease using samples collected from children <5 years living in different states of Brazil. Blood samples collected by finger prick on filter paper were sent to the test laboratory by a central laboratory to confirm results obtained previously. Ten percent of negative samples, all doubtful and all positive samples were received. Commercial reagents, IgG indirect immunofluorescence, enzyme immunoassay, and a recently introduced TESAcruzi test were used. From 8788 samples, 163 (1.85%) were reactive by IgG-ELISA and 312 (3.55%) by IgG IIF. From these, 77 (0.87%) were reactive in the TESAcruzi test. The results had high clinical value to identify those truly infected.
dc.description.indexMEDLINE
dc.identifier.citationACTA TROPICA, v.117, n.1, p.10-13, 2011
dc.identifier.doi10.1016/j.actatropica.2010.08.018
dc.identifier.issn0001-706X
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/23074
dc.language.isoeng
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofActa Tropica
dc.rightsrestrictedAccess
dc.rights.holderCopyright ELSEVIER SCIENCE BV
dc.subjectDiagnosis
dc.subjectChagas disease
dc.subjectTESAcruzi
dc.subject.otherchronic chagas-disease
dc.subject.otherserological diagnosis
dc.subject.otherassay
dc.subject.otherinfection
dc.subject.otherdiscovery
dc.subject.wosParasitology
dc.subject.wosTropical Medicine
dc.titleWestern blotting method (TESAcruzi) as a supplemental test for confirming the presence of anti-Trypanosoma cruzi antibodies in finger prick blood samples from children aged 0-5 years in Brazil
dc.typearticle
dc.type.categoryoriginal article
dc.type.versionpublishedVersion
dspace.entity.typePublication
hcfmusp.author.externalLUQUETTI, Alejandro O.:Univ Fed Goias, Inst Trop Pathol & Publ Hlth, Lab Chagas Dis, BR-74605050 Goiania, Go, Brazil
hcfmusp.author.externalPRATA, Aluisio:Sch Med Triangulo Mineiro, BR-38025180 Uberaba, MG, Brazil
hcfmusp.citation.scopus7
hcfmusp.contributor.author-fmusphcAMANDA FARAGE FRADE BARROS
hcfmusp.contributor.author-fmusphcANTONIO WALTER FERREIRA
hcfmusp.description.beginpage10
hcfmusp.description.endpage13
hcfmusp.description.issue1
hcfmusp.description.volume117
hcfmusp.origemWOS
hcfmusp.origem.pubmed20858452
hcfmusp.origem.scopus2-s2.0-78049466659
hcfmusp.origem.wosWOS:000284785600003
hcfmusp.publisher.cityAMSTERDAM
hcfmusp.publisher.countryNETHERLANDS
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hcfmusp.scopus.lastupdate2024-05-17
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