https://observatorio.fm.usp.br/handle/OPI/10521
DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | SANTOS, Maria Mercês | - |
dc.contributor.author | TANNURI, Ana Cristina Aoun | - |
dc.contributor.author | COELHO, Maria Cecilia Mendonça | - |
dc.contributor.author | GONÇALVES, Josiane de Oliveira | - |
dc.contributor.author | SERAFINI, Suellen | - |
dc.contributor.author | SILVA, Luiz Fernando Ferraz da | - |
dc.contributor.author | TANNURI, Uenis | - |
dc.date.accessioned | 2015-09-11T17:47:17Z | - |
dc.date.available | 2015-09-11T17:47:17Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | CLINICS, v.70, n.5, p.373-379, 2015 | - |
dc.identifier.issn | 1807-5932 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/10521 | - |
dc.description.abstract | OBJECTIVE: Intestinal ischemia-reperfusion injury occurs in several clinical conditions and after intestinal transplantation. The aim of the present study was to investigate the phenomena of apoptosis and cell proliferation in a previously described intestinal ischemia-reperfusion injury autograft model using immunohistochemical markers. The molecular mechanisms involved in ischemia-reperfusion injury repair were also investigated by measuring the expression of the early activation genes c-fos and c-jun, which induce apoptosis and cell proliferation. MATERIALS AND METHODS: Thirty adult male Wistar rats were subjected to surgery for a previously described ischemia-reperfusion model that preserved the small intestine, the cecum and the ascending colon. Following reperfusion, the cecum was harvested at different time points as a representative segment of the intestine. The rats were allocated to the following four subgroups according to the reperfusion time: subgroup 1: 5 min; subgroup 2: 15 min; subgroup 3: 30 min; and subgroup 4: 60 min. A control group of cecum samples was also collected. The expression of c-fos, c-jun and immunohistochemical markers of cell proliferation and apoptosis (Ki67 and TUNEL, respectively) was studied. RESULTS: The expression of both c-fos and c-jun in the cecum was increased beginning at 5 min after ischemia-reperfusion compared with the control. The expression of c-fos began to increase at 5 min, peaked at 30 min, and exhibited a declining tendency at 60 min after reperfusion. A progressive increase in c-jun expression was observed. Immunohistochemical analyses confirmed these observations. CONCLUSION: The early activation of the c-fos and c-jun genes occurred after intestinal ischemia-reperfusion injury, and these genes can act together to trigger cell proliferation and apoptosis. | - |
dc.language.iso | eng | - |
dc.publisher | Faculdade de Medicina / USP | - |
dc.relation.ispartof | Clinics | - |
dc.rights | openAccess | - |
dc.subject | Ischemia-reperfusion injury | - |
dc.subject | Immediate early genes | - |
dc.subject | Activator protein 1 | - |
dc.subject | Small intestine | - |
dc.subject | C-fos | - |
dc.subject | C-jun gene | - |
dc.subject.other | APOPTOSIS | - |
dc.subject.other | INJURY | - |
dc.subject.other | ISCHEMIA/REPERFUSION | - |
dc.subject.other | EPITHELIUM | - |
dc.title | Immediate expression of c-fos and c-jun mRNA in a model of intestinal autotransplantation and ischemia-reperfusion in situ | - |
dc.type | article | - |
dc.rights.holder | Copyright Faculdade de Medicina / USP | - |
dc.identifier.doi | 10.6061/clinics/2015(05)12 | - |
dc.identifier.pmid | 26039956 | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.description.beginpage | 373 | - |
hcfmusp.description.endpage | 379 | - |
hcfmusp.description.issue | 5 | - |
hcfmusp.description.volume | 70 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | 2-s2.0-84930519802 | - |
hcfmusp.origem.id | WOS:000355800500012 | - |
hcfmusp.origem.id | SCIELO:S1807-59322015000500373 | - |
hcfmusp.publisher.city | São Paulo | - |
hcfmusp.publisher.country | BRAZIL | - |
hcfmusp.relation.reference | Attuwaybi BO, 2003, J SURG RES, V115, P48, DOI 10.1016/S0022-4804(03)00313-5 | - |
hcfmusp.relation.reference | Goncalves JO, 2014, BRAZ J MED BIOL RES, V47, P850, DOI 10.1590/1414-431X20143679 | - |
hcfmusp.relation.reference | Hassoun HT, 2003, J SURG RES, V115, P121, DOI 10.1016/S0022-4808(03)00298-1 | - |
hcfmusp.relation.reference | Huppertz B, 1999, ANAT EMBRYOL, V200, P1, DOI 10.1007/s004290050254 | - |
hcfmusp.relation.reference | Ikeda H, 1998, GUT, V42, P530 | - |
hcfmusp.relation.reference | Li Y, 2003, AM J RESP CELL MOL, V29, P779, DOI 10.1165/rcmb.2003-0087RC | - |
hcfmusp.relation.reference | Livak KJ, 2001, METHODS, V25, P402, DOI 10.1006/meth.2001.1262 | - |
hcfmusp.relation.reference | Noda T, 1998, AM J PHYSIOL-GASTR L, V274, pG270 | - |
hcfmusp.relation.reference | Onishi I, 1999, TRANSPLANT P, V31, P1077, DOI 10.1016/S0041-1345(98)01912-5 | - |
hcfmusp.relation.reference | Pasternak BA, 2010, PEDIATR TRANSPLANT, V14, P72, DOI 10.1111/j.1399-3046.2008.01125.x | - |
hcfmusp.relation.reference | Santos MM, 2009, TRANSPL P, V41, P3617, DOI 10.1016/j.transproceed.2009.05.037 | - |
hcfmusp.relation.reference | Shah KA, 1997, INT J EXP PATHOL, V78, P355, DOI 10.1046/j.1365-2613.1997.420369.x | - |
hcfmusp.relation.reference | Shima Y, 2006, J PEDIATR SURG, V41, P830, DOI 10.1016/j.pedsurg.2005.12.025 | - |
hcfmusp.relation.reference | Taguchi T, 2000, TRANSPLANT P, V32, P1279, DOI 10.1016/S0041-1345(00)01224-0 | - |
hcfmusp.relation.reference | Wieland E, 2000, CLIN BIOCHEM, V33, P285, DOI 10.1016/S0009-9120(00)00070-9 | - |
hcfmusp.relation.reference | 1994, [No title captured], pS73 | - |
dc.description.index | MEDLINE | - |
dc.identifier.eissn | 1980-5322 | - |
hcfmusp.citation.scopus | 11 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCG Artigos e Materiais de Revistas Científicas - FM/MPE Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICr Artigos e Materiais de Revistas Científicas - LIM/05 Artigos e Materiais de Revistas Científicas - LIM/26 Artigos e Materiais de Revistas Científicas - LIM/30 |
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art_SANTOS_Immediate_expression_of_cfos_and_cjun_mRNA_in_2015.PDF | publishedVersion (English) | 1.83 MB | Adobe PDF | View/Open |
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