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https://observatorio.fm.usp.br/handle/OPI/1112
Title: | QUANTIFICATION OF TRYPTOPHAN IN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
Authors: | PINHATI, Renata Romanholi; POLONINI, Hudson Caetano; BRANDAO, Marcos Antonio Fernandes; RAPOSO, Nadia Rezende Barbosa; CORCHS, Felipe; GATTAZ, Wagner Farid |
Citation: | QUIMICA NOVA, v.35, n.3, p.623-626, 2012 |
Abstract: | A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPS Artigos e Materiais de Revistas Científicas - HC/IPq Artigos e Materiais de Revistas Científicas - LIM/27 |
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art_CORCHS_QUANTIFICATION_OF_TRYPTOPHAN_IN_PLASMA_BY_HIGH_PERFORMANCE_2012.PDF | publishedVersion (English) | 269.07 kB | Adobe PDF | ![]() View/Open |
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