https://observatorio.fm.usp.br/handle/OPI/14405
Title: | Prevalence of human papillomavirus types and variants and p16(INK4a) expression in head and neck squamous cells carcinomas in Sao Paulo, Brazil |
Authors: | BETIOL, Julio C.; SICHERO, Laura; COSTA, Henrique O. de Olival; MATOS, Leandro L. de; ANDREOLI, Maria A.; FERREIRA, Silvaneide; FARAJ, Sheila F.; MELLO, Evandro S. de; SOBRINHO, Joao S.; BRANDAO, Lenine G.; CERNEA, Claudio R.; KULCSAR, Marco A.; PINTO, Fabio R.; GONCALVES, Antonio J.; MENEZES, Marcelo B.; SILVA, Leonardo; ROSSI, Lia M.; NUNES, Rafaella A. Lima; TERMINI, Lara; VILLA, Luisa L. |
Citation: | INFECTIOUS AGENTS AND CANCER, v.11, article ID 20, 7p, 2016 |
Abstract: | Background: Human papillomavirus (HPV) prevalence in head and neck squamous cell carcinomas (HNSCC) diverges geographically. The reliability of using p16(INK4a) expression as a marker of viral infection is controversial in HNSCC. We evaluated HPV types and HPV-16 variants prevalence, and p16(INK4a) expression in HNSCC specimens provided by two different Institutions in Sao Paulo. Methods: HPV DNA from formalin-fixed specimens was accessed by Inno-LiPA, HPV-16 variants by PCR-sequencing, and p16(INK4a) protein levels by immunohistochemistry. Results: Overall, HPV DNA was detected among 19.4 % of the specimens (36/186). Viral prevalence was higher in the oral cavity (25.0 %, 23/92) then in other anatomical sites (oropharynx 14,3 %, larynx 13.7 %) when samples from both Institutions were analyzed together. HPV prevalence was also higher in the oral cavity when samples from both Institutions were analyzed separately. HPV-16 was the most prevalent type identified in 69.5 % of the HPV positive smaples and specimens were assigned into Asian-American (57.2 %) or European (42.8 %) phylogenetic branches. High expression of p16(INK4a) was more common among HPV positive tumors. Conclusion: Our results support a role for HPV-16 in a subset of HNSCC. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCG Artigos e Materiais de Revistas Científicas - FM/MDR Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICESP Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/14 Artigos e Materiais de Revistas Científicas - LIM/24 Artigos e Materiais de Revistas Científicas - LIM/28 Artigos e Materiais de Revistas Científicas - ODS/03 |
File | Description | Size | Format | |
---|---|---|---|---|
art_BETIOL_Prevalence_of_human_papillomavirus_types_and_variants_and_2016.PDF | publishedVersion (English) | 495.32 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.