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https://observatorio.fm.usp.br/handle/OPI/16324
Title: | Evaluation of Leishmania (Leishmania) infantum excreted-secreted antigens for detection of canine leishmaniasis |
Authors: | PINEDO-CANCINO, Viviana; LAURENTI, Marcia Dalastra; KESPER, Norival; UMEZAWA, Eufrosina Setsu |
Citation: | ACTA TROPICA, v.161, p.41-43, 2016 |
Abstract: | The efficacy of tests with L (L.) infantum excreted-secreted antigens (ESA) to detect canine leishmaniasis (CanL) was evaluated using immunoblotting (ESA-blot), ELISA (ESA-ELISA) and ELISA with alkaline extract from promastigotes (PAE). Of one hundred fifty-five domestic dogs tested, 100 were suspected of CanL, 23 had other diseases and 32 were healthy. Sera from the dogs suspected of CanL were tested by immunohistochemistry (IHC), and 54% were confirmed to be infected by L (L.) infantum (38 symptomatic and 16 asymptomatic). Of these, 100% were positive by ESA-blot, ESA-ELISA and PAE-ELISA. In the ESA blot their sera recognized polypeptides in the 26.5-31.5 kDa region. Of the 46% of dogs with negative IHC, 44-53% tested positive in all three tests irrespective of clinical status. The twenty-three dogs with other diseases were negative by ESA-blot, but sera from 9% and 26% of them reacted with ESA-ELISA and PAE-ELISA, respectively. The 32 healthy dogs were negative in all the tests. ESA-blot showed good correlation with IHC in the detection of CanL and a high specificity index. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPR Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/49 Artigos e Materiais de Revistas Científicas - LIM/50 |
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