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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | DANTAS, Katia Cristina | - |
dc.contributor.author | FREITAS, Roseli S. | - |
dc.contributor.author | MOREIRA, Adriana Pardini Vicentini | - |
dc.contributor.author | SILVA, Marcos Vinicius da | - |
dc.contributor.author | BENARD, Gil | - |
dc.contributor.author | VASCONCELLOS, Cidia | - |
dc.contributor.author | CRIADO, Paulo Ricardo | - |
dc.date.accessioned | 2013-09-23T16:38:10Z | - |
dc.date.available | 2013-09-23T16:38:10Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | ANAIS BRASILEIROS DE DERMATOLOGIA, v.88, n.1, p.141-143, 2013 | - |
dc.identifier.issn | 0365-0596 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/1912 | - |
dc.description.abstract | The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis. | - |
dc.description.abstract | O objetivo do estudo consistiu em detectar seqüências no ADNr e as suas regiões no Histoplasma capsulatum, que pudessem ser consideradas espécie-específicas e usadas como método molecular para o diagnóstico pela técnica da reação em cadeia da polimerase aninhada ("nested PCR") com seqüências específicas ("primers") para H. capsulatum: regiões 18S ADNr (HC18), 100kDa (HC100) e a seqüência 5.8 S ADNr-ITS (HC5.8). A "nested PCR" com as seqüências HC18, HC100 e HC5.8 resultaram em 100% de especificidade. Os ensaios moleculares podem aumentar a especificidade, sensibilidade e rapidez na diagnose da Histoplasmose. | - |
dc.description.sponsorship | Faculty of Medicine of the University of Sao Paulo (FMUSP) | - |
dc.language.iso | eng | - |
dc.publisher | SOC BRASILEIRA DERMATOLOGIA | - |
dc.relation.ispartof | Anais Brasileiros de Dermatologia | - |
dc.rights | openAccess | - |
dc.subject | AIDS-related opportunistic infections | - |
dc.subject | Histoplasma | - |
dc.subject | HIV seropositivity | - |
dc.subject | Molecular diagnostic techniques | - |
dc.subject | Polymerase chain reaction | - |
dc.subject | Infecções oportunistas relacionadas com a AIDS | - |
dc.subject | Reação em cadeia da polimerase | - |
dc.subject | Soropositividade para HIV | - |
dc.subject | Técnicas de diagnóstico molecular | - |
dc.subject.other | assays | - |
dc.title | The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients | - |
dc.title.alternative | Uso da Reação em Cadeia da Polimerase aninhada (PCR aninhada) para o diagnóstico precoce da infecção pelo Histoplasma capsulatum no soro e no sangue total de pacientes HIV positivos | - |
dc.type | article | - |
dc.rights.holder | Copyright SOC BRASILEIRA DERMATOLOGIA | - |
dc.identifier.doi | 10.1590/S0365-05962013000100025 | - |
dc.identifier.pmid | 23539023 | - |
dc.subject.wos | Dermatology | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | MOREIRA, Adriana Pardini Vicentini:Fed Univ Sao Paulo UNIFESP, Sao Paulo, Brazil; State Hlth Secretariat, Adolfo Lutz Inst, Sao Paulo, Brazil | - |
hcfmusp.author.external | SILVA, Marcos Vinicius da:Univ Sao Paulo FMUSP, Fac Med Med, Sao Paulo, Brazil; Emilio Ribas Infectol Inst, Sao Paulo, Brazil | - |
hcfmusp.author.external | VASCONCELLOS, Cidia:Univ Sao Paulo FMUSP, Fac Med, Stricto Sensu Postgrad Course, Sao Paulo, Brazil; Insitute State Publ Employees IAMSPE, Sao Paulo, Brazil | - |
hcfmusp.description.beginpage | 141 | - |
hcfmusp.description.endpage | 143 | - |
hcfmusp.description.issue | 1 | - |
hcfmusp.description.volume | 88 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000316807000025 | - |
hcfmusp.origem.id | 2-s2.0-84875407585 | - |
hcfmusp.origem.id | SCIELO:S0365-05962013000100141 | - |
hcfmusp.publisher.city | RIO DE JANEIRO RJ | - |
hcfmusp.publisher.country | BRAZIL | - |
hcfmusp.relation.reference | Bialek R, 2002, J CLIN MICROBIOL, V40, P1644, DOI 10.1128/JCM.40.5.1644-1647.2002 | - |
hcfmusp.relation.reference | Freitas RS, 2009, J VENOM ANIM TOXINS, V15, P278, DOI 10.1590/S1678-91992009000200010 | - |
hcfmusp.relation.reference | Fugita S, 2001, J CLIN MICROBIOL, V39, P3617 | - |
hcfmusp.relation.reference | Kauffman CA, 2007, CLIN MICROBIOL REV, V20, P115, DOI 10.1128/CMR.00027-06 | - |
hcfmusp.relation.reference | Maubon D, 2007, DIAGN MICR INFEC DIS, V58, P441, DOI 10.1016/j.diagmicrobio.2007.03.008 | - |
hcfmusp.relation.reference | WHEAT J, 1993, ANN INTERN MED, V118, P610 | - |
hcfmusp.relation.reference | Wheat LJ, 2002, DIAGN MICR INFEC DIS, V43, P29, DOI 10.1016/S0732-8893(02)00367-X | - |
dc.description.index | MEDLINE | - |
hcfmusp.remissive.sponsorship | FMUSP | - |
hcfmusp.citation.scopus | 22 | - |
hcfmusp.scopus.lastupdate | 2024-04-12 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MDT Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/05 Artigos e Materiais de Revistas Científicas - LIM/53 Artigos e Materiais de Revistas Científicas - LIM/56 Artigos e Materiais de Revistas Científicas - ODS/03 |
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art_DANTAS_The_use_of_nested_Polymerase_Chain_Reaction_(nested_2013.PDF | publishedVersion (English) | 78.26 kB | Adobe PDF | View/Open |
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