Use este identificador para citar ou linkar para este item: https://observatorio.fm.usp.br/handle/OPI/20105
Título: Determination of Fetal RHD Genotype Including the RHD Pseudogene in Maternal Plasma
Autor(es): ZIZA, Karen ChinocaLIAO, Adolfo WenjawDEZAN, MarciaDINARDO, Carla LuanaJENS, EduardoFRANCISCO, Rossana Pulcineli VieiraMENDRONE JUNIOR, AlfredoZUGAIB, MarceloLEVI, Jose Eduardo
Parte de: JOURNAL OF CLINICAL LABORATORY ANALYSIS, v.31, n.3, article ID e22052, 6p, 2017
Resumo: Objective: To examine the accuracy of fetal RHD genotype and RHD pseudogene determination in a multiethnical population. Methods: Prospective study involving D-negative pregnant women. Cell-free DNA was extracted from 1 ml of maternal plasma by an automated system (MagNA Pure Compact, Roche) and real-time PCR was performed in triplicate targeting the RHD gene exons 5 and 7. Inconclusive samples underwent RHD pseudogene testing by real-time PCR analysis employing novel primers and probe. Results: A positive result was observed in 128/185 (69.2%) samples and negative in 50 (27.0%). Umbilical cord blood phenotype confirmed all cases with a positive or negative PCR result. Seven (3.8%) cases were found inconclusive (exon 7 amplification only) and RHD pseudogene testing with both conventional and real-time PCR demonstrated a positive result in five of them, while two samples were also RHD pseudogene negative. Conclusion: Real-time PCR targeting RHD exons 5 and 7 simultaneously in maternal plasma is an accurate method for the diagnosis of fetal D genotype in our population. The RHD pseudogene real-time PCR assay is feasible and is particularly useful in populations with a high prevalence of this allele. (C) 2016 Wiley Periodicals, Inc.
Aparece nas coleções:

Artigos e Materiais de Revistas Científicas - FM/MOG
Departamento de Obstetrícia e Ginecologia - FM/MOG

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

Artigos e Materiais de Revistas Científicas - LIM/13
LIM/13 - Laboratório de Genética e Cardiologia Molecular

Artigos e Materiais de Revistas Científicas - LIM/52
LIM/52 - Laboratório de Virologia

Artigos e Materiais de Revistas Científicas - LIM/57
LIM/57 - Laboratório de Fisiologia Obstétrica


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