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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorZIZA, Karen Chinoca-
dc.contributor.authorLIAO, Adolfo Wenjaw-
dc.contributor.authorDEZAN, Marcia-
dc.contributor.authorDINARDO, Carla Luana-
dc.contributor.authorJENS, Eduardo-
dc.contributor.authorFRANCISCO, Rossana Pulcineli Vieira-
dc.contributor.authorMENDRONE JUNIOR, Alfredo-
dc.contributor.authorZUGAIB, Marcelo-
dc.contributor.authorLEVI, Jose Eduardo-
dc.date.accessioned2017-06-09T15:36:07Z-
dc.date.available2017-06-09T15:36:07Z-
dc.date.issued2017-
dc.identifier.citationJOURNAL OF CLINICAL LABORATORY ANALYSIS, v.31, n.3, article ID e22052, 6p, 2017-
dc.identifier.issn0887-8013-
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/20105-
dc.description.abstractObjective: To examine the accuracy of fetal RHD genotype and RHD pseudogene determination in a multiethnical population. Methods: Prospective study involving D-negative pregnant women. Cell-free DNA was extracted from 1 ml of maternal plasma by an automated system (MagNA Pure Compact, Roche) and real-time PCR was performed in triplicate targeting the RHD gene exons 5 and 7. Inconclusive samples underwent RHD pseudogene testing by real-time PCR analysis employing novel primers and probe. Results: A positive result was observed in 128/185 (69.2%) samples and negative in 50 (27.0%). Umbilical cord blood phenotype confirmed all cases with a positive or negative PCR result. Seven (3.8%) cases were found inconclusive (exon 7 amplification only) and RHD pseudogene testing with both conventional and real-time PCR demonstrated a positive result in five of them, while two samples were also RHD pseudogene negative. Conclusion: Real-time PCR targeting RHD exons 5 and 7 simultaneously in maternal plasma is an accurate method for the diagnosis of fetal D genotype in our population. The RHD pseudogene real-time PCR assay is feasible and is particularly useful in populations with a high prevalence of this allele. (C) 2016 Wiley Periodicals, Inc.-
dc.language.isoeng-
dc.publisherWILEY-
dc.relation.ispartofJournal of Clinical Laboratory Analysis-
dc.rightsrestrictedAccess-
dc.subjectDNA-
dc.subjectanalysis-
dc.subjectgenotyping techniques-
dc.subjectprenatal diagnosis-
dc.subjectmethods-
dc.subjectRhD Blood group system-
dc.subjectsensitivity and specificity-
dc.subject.otherbrazilian blood-donors-
dc.subject.otherpregnant-women-
dc.subject.otherdna-
dc.subject.otherpopulation-
dc.subject.otheridentification-
dc.subject.othermetaanalysis-
dc.subject.otherprophylaxis-
dc.subject.otherexperience-
dc.subject.otherphenotypes-
dc.subject.otherafricans-
dc.titleDetermination of Fetal RHD Genotype Including the RHD Pseudogene in Maternal Plasma-
dc.typearticle-
dc.rights.holderCopyright WILEY-
dc.identifier.doi10.1002/jcla.22052-
dc.identifier.pmid27595845-
dc.subject.wosMedical Laboratory Technology-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-
hcfmusp.description.articlenumbere22052-
hcfmusp.description.issue3-
hcfmusp.description.volume31-
hcfmusp.origemWOS-
hcfmusp.origem.idWOS:000401538800008-
hcfmusp.origem.id2-s2.0-84992612730-
hcfmusp.publisher.cityHOBOKEN-
hcfmusp.publisher.countryUSA-
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dc.description.indexMEDLINE-
dc.identifier.eissn1098-2825-
hcfmusp.citation.scopus2-
hcfmusp.scopus.lastupdate2022-06-17-
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MOG
Departamento de Obstetrícia e Ginecologia - FM/MOG

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

Artigos e Materiais de Revistas Científicas - LIM/13
LIM/13 - Laboratório de Genética e Cardiologia Molecular

Artigos e Materiais de Revistas Científicas - LIM/52
LIM/52 - Laboratório de Virologia

Artigos e Materiais de Revistas Científicas - LIM/57
LIM/57 - Laboratório de Fisiologia Obstétrica


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