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Title: Luminescent material based on the [Eu(TTA)(3)(H2O)(2)] complex incorporated into modified silica particles for biological applications
Authors: LOURENCO, Ana Valeria S.KODAIRA, Claudia A.RAMOS-SANCHEZ, Eduardo M.FELINTO, Maria Claudia F. C.GOTO, HiroGIDLUND, MagnusMALTA, Oscar L.BRITO, Hermi F.
Citation: JOURNAL OF INORGANIC BIOCHEMISTRY, v.123, p.11-17, 2013
Abstract: Amino-functionalized luminescent silica particles were investigated for use in immunoassays. The particles were prepared by the Stober method where the [Eu(TTA)(3)(H2O)(2)] complex (TTA: 3-thenoyltrifluoroacetonate) was incorporated into silica particles during the hydrolysis and condensation of TEOS: tetraethylorthosilicate. Then, the amino groups were introduced in the particle surface using APTS: 3-aminopropyltriethoxisilane. The resulting particles were characterized by scanning electron microscopy (SEM), X ray diffraction (XRD) and photoluminescence spectroscopy. In order to demonstrate the viability of the use of luminescent particles as optical markers, an enzyme-substrate reaction was performed using HRP: horseradish peroxidase. It was possible to verify the binding of HRP-oxidized LDL (low density lipoprotein) and anti-oxLDL antibody-luminescent silica particles through the evaluation of the presence of HRP. The bioassay data open a broad field for the development of protein-tagged luminescent particles for use in biomedical sciences.
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Artigos e Materiais de Revistas Científicas - FM/MPR
Departamento de Medicina Preventiva - FM/MPR

Artigos e Materiais de Revistas Científicas - LIM/38
LIM/38 - Laboratório de Epidemiologia e Imunobiologia

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