Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/2284
Title: | N-acetylcysteine attenuates renal alterations induced by senescence in the rat |
Authors: | SHIMIZU, Maria Heloisa M.; VOLPINI, Rildo A.; BRAGANCA, Ana Carolina de; CAMPOS, Renata; CANALE, Daniele; SANCHES, Talita R.; ANDRADE, Lucia; SEGURO, Antonio C. |
Citation: | EXPERIMENTAL GERONTOLOGY, v.48, n.2, p.298-303, 2013 |
Abstract: | The aim of this study was to evaluate the effects of N-acetylcysteine (NAC) on renal function, as well as on sodium and water transporters, in the kidneys of aged rats. Normal, 8-month-old male Wistar rats were treated (n = 6) or not (n = 6) with NAC (600 mg/L in drinking water) and followed for 16 months. At the end of the follow-up period, we determined inulin clearance, serum thiobarbituric acid reactive substances (TBARS), serum cholesterol, and urinary phosphate excretion. In addition, we performed immunohistochemical staining for p53 and for ED-1-positive cells (macrophages/monocytes), together with Western blotting of kidney tissue for NKCC2, aquaporin 2 (AQP2), urea transporter A1 (UT-A1) and Klotho protein. At baseline, the two groups were similar in terms of creatinine clearance, proteinuria, cholesterol, and TBARS. At the end of the follow-up period, NAC-treated rats presented greater inulin clearance and reduced proteinuria, as well as lower serum cholesterol, serum TBARS, and urinary phosphate excretion, in comparison with untreated rats. In addition, NAC-treated rats showed upregulated expression of NKCC2, AQP2, and UT-A1; elevated Klotho protein expression, low p53 expression, and few ED-1 positive cells. In conclusion, we attribute these beneficial effects of NAC (the significant improvements in inulin clearance and in the expression of NKCC2, AQP2, and UT-A1) to its ability to decrease oxidative stress, inhibit p53 expression, minimize kidney inflammation, and stimulate Klotho expression. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCM Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/12 Artigos e Materiais de Revistas Científicas - ODS/06 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_SHIMIZU_N_acetylcysteine_attenuates_renal_alterations_induced_by_senescence_2013.PDF Restricted Access | publishedVersion (English) | 638.19 kB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.