Effects of melatonin on histomorphology and on the expression of steroid receptors, VEGF, and PCNA in ovaries of pinealectomized female rats

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author ROMEU, Lucrecia Regina G.
MOTTA, Eduardo Leme A. da
MAGANHIN, Carla C.
OSHIMA, Celina T. F.
FONSECA, Marcelle C.
BARRUECO, Karina F.
SIMOES, Ricardo S.
PELLEGRINO, Renata
BARACAT, Edmund C. FMUSP-HC
SOARES-JUNIOR, Jose M.
dc.date.issued 2011
dc.identifier.citation FERTILITY AND STERILITY, v.95, n.4, p.1379-1384, 2011
dc.identifier.issn 0015-0282
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/23215
dc.description.abstract Objective: To evaluate the effect of melatonin both on the ovaries of pinealectomized female rats through histomorphometric analysis and on steroid receptors, proliferating cell nuclear antigen (PCNA), and vascular endothelial growth factor (VEGF) expression. Design: Experimental study. Setting: Federal University of Sao Paulo, Brazil. Animal(s): Forty female rats. Intervention(s): Forty rats were divided equally into four groups: GI-vehicle without surgery; GII-surgery without removal of the pineal gland (sham); GIII-pinealectomized with vehicle; and GIV-pinealectomized with melatonin treatment. After treatment for 3 consecutive months, the animals were killed and their ovaries removed for analysis. Main Outcome Measure(s): Estrogen and progesterone receptors, histologic and immunohistochemical analysis. Result(s): The GIII samples presented signals of proliferation on ovarian surface epithelium and interstitial cells as well as high expressions of PCNA and VEGF in those structures compared with GI, GII, and GIV. Also, the levels of progesterone receptor (fmol/g) in ovaries of GIII (250.6 +/- 32.4) were significantly lower than in those of GI (429.0 +/- 23,8), GII (442.3 +/- 30.2), and GIV (564.1 +/- 78.7). The levels of progesterone in GIII were superior to those in GI, GII, and GIV. Conclusion(s): Our findings suggest that melatonin may attenuate proliferation in ovarian structures and increase the number of luteal bodies as well as the levels of progesterone receptor. (Fertil Steril (R) 2011;95:1379-84. (C)2011 by American Society for Reproductive Medicine.)
dc.description.sponsorship · Sao Paulo Research Foundation (FAPESP) [2006/60412-7, 2007/54398-4]
dc.language.iso eng
dc.publisher ELSEVIER SCIENCE INC
dc.relation.ispartof Fertility and Sterility
dc.rights restrictedAccess
dc.subject Melatonin; ovary; VEGF; PCNA; rats
dc.subject.other endothelial growth-factor; breast-cancer; progesterone-receptor; alzheimers-disease; estrous-cycle; hormone; cells; inhibition; modulation; morphology
dc.title Effects of melatonin on histomorphology and on the expression of steroid receptors, VEGF, and PCNA in ovaries of pinealectomized female rats
dc.type article
dc.rights.holder Copyright ELSEVIER SCIENCE INC
dc.description.group LIM/58
dc.identifier.doi 10.1016/j.fertnstert.2010.04.042
dc.identifier.pmid 20605140
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author BARACAT, Edmund C.:FM:MOG
hcfmusp.author.external · ROMEU, Lucrecia Regina G.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
· MOTTA, Eduardo Leme A. da:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
· MAGANHIN, Carla C.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
· OSHIMA, Celina T. F.:Univ Fed Sao Paulo, Pathol Sect, Sao Paulo, Brazil
· FONSECA, Marcelle C.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
· BARRUECO, Karina F.:Univ Fed Sao Paulo, Dept Psychobiol, Sao Paulo, Brazil
· SIMOES, Ricardo S.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
· PELLEGRINO, Renata:Univ Fed Sao Paulo, Dept Psychobiol, Sao Paulo, Brazil
· SOARES-JUNIOR, Jose M.:Univ Fed Sao Paulo, Dept Gynecol, Sao Paulo, Brazil
hcfmusp.origem.id 2-s2.0-79952439370
hcfmusp.origem.id WOS:000288010900031
hcfmusp.publisher.city NEW YORK
hcfmusp.publisher.country USA
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dc.description.index MEDLINE
hcfmusp.citation.scopus 31
hcfmusp.affiliation.country Brasil
hcfmusp.scopus.lastupdate 2021-08-27


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