Effect of neurotrophic factor, MDP, on rats' nerve regeneration

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP
dc.contributor.author FORNAZARI, A. A. FMUSP-HC
REZENDE, M. R. de FMUSP-HC
MATTAR JR., R. FMUSP-HC
TAIRA, R. I. FMUSP-HC
SANTOS, G. B. dos FMUSP-HC
PAULOS, R. G. FMUSP-HC
dc.date.issued 2011
dc.identifier.citation BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, v.44, n.4, p.327-331, 2011
dc.identifier.issn 0100-879X
dc.identifier.uri http://observatorio.fm.usp.br/handle/OPI/23266
dc.description.abstract Our objective was to determine the immune-modulating effects of the neurotrophic factor N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) on median nerve regeneration in rats. We used male Wistar rats (120-140 days of age, weighing 250-332 g) and compared the results of three different techniques of nerve repair: 1) epineural neurorrhaphy using sutures alone (group S - 10 rats), 2) epineural neurorrhaphy using sutures plus fibrin tissue adhesive (FTA; group SF - 20 rats), and 3) sutures plus FTA, with MDP added to the FTA (group SFM - 20 rats). Functional assessments using the grasp test were performed weekly for 12 weeks to identify recovery of flexor muscle function in the fingers secondary to median nerve regeneration. Histological analysis was also utilized. The total number and diameter of myelinated fibers were determined in each proximal and distal nerve segment. Two indices, reported as percentage, were calculated from these parameters, namely, the regeneration index and the diameter change index. By the 8th week, superiority of group SFM over group S became apparent in the grasping test (P = 0.005). By the 12th week, rats that had received MDP were superior in the grasping test compared to both group S (P < 0.001) and group SF (P = 0.001). Moreover, group SF was better in the grasping test than group S (P = 0.014). However, no significant differences between groups were identified by histological analysis. In the present study, rats that had received MDP obtained better function, in the absence of any significant histological differences.
dc.description.sponsorship · FAPESP
dc.language.iso eng
dc.publisher ASSOC BRAS DIVULG CIENTIFICA
dc.relation.ispartof Brazilian Journal of Medical and Biological Research
dc.rights openAccess
dc.subject Grasping test; Neurotrophic factor; N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP)
dc.subject.other wallerian degeneration; muramyl dipeptide; sciatic-nerve; macrophages; repair
dc.title Effect of neurotrophic factor, MDP, on rats' nerve regeneration
dc.type article
dc.rights.holder Copyright ASSOC BRAS DIVULG CIENTIFICA
dc.description.group LIM/41
dc.identifier.pmid 21344131
dc.type.category original article
dc.type.version publishedVersion
hcfmusp.author FORNAZARI, A. A.:FM:
hcfmusp.author REZENDE, M. R. de:HC:IOT
hcfmusp.author MATTAR JR., R.:FM:MOT
hcfmusp.author TAIRA, R. I.:HC:FMUSP
hcfmusp.author SANTOS, G. B. dos:HC:IOT
hcfmusp.author PAULOS, R. G.:HC:FFM
hcfmusp.origem.id WOS:000289368400009
hcfmusp.origem.id 2-s2.0-79955364455
hcfmusp.publisher.city SAO PAULO
hcfmusp.publisher.country BRAZIL
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dc.description.index MEDLINE
dc.identifier.eissn 1678-4510
hcfmusp.citation.scopus 2
hcfmusp.scopus.lastupdate 2021-07-14


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