Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/23461
Title: | Cell Therapy for Fibrotic Interstitial Pulmonary Disease: Experimental Study |
Authors: | CABRAL, Rosa M.; BRANCO, Erika; RIZZO, Marcia Dos S.; FERREIRA, Guilherme J.; GREGORES, Guilherme B.; SAMOTO, Vivian Y.; STOPIGLIA, Angelo J.; MAIORKA, Paulo C.; FIORETTO, Emerson T.; CAPELOZZI, Vera L.; BORGES, Joao B.; GOMES, Susimeire; BERALDO, Marcelo A.; CARVALHO, Carlos R. R.; MIGLINO, Maria A. |
Citation: | MICROSCOPY RESEARCH AND TECHNIQUE, v.74, n.10, p.957-962, 2011 |
Abstract: | Parte superior do formulario Digite um texto ou endereco de um site ou traduza um documento. The aim of this study is to evaluate the histological changes in lung parenchyma of pigs affected by interstitial lung disease induced after the infusion of bone marrow mononuclear cells (BMMCs). Ten female swines were submitted to pulmonary fibrosis induced by a single dose of intratracheal bleomicine sulfate. Animals were arranged into two groups: Group 1: induced-disease control and Group 2: cell therapy using BMMCs. Both groups were clinically evaluated for 180 days. High-resolution computed tomography (HRCT) was performed at 90 and 180 days. BMMC sampling was performed in cell therapy group at 90 days. Euthanasia was performed, and samples were collected for histology and immunohistochemistry. The 90-days HRCT demonstrated typical interstitial lesions in pulmonary parenchyma similarly to human disease. The 180-days HRCT in Group 1 demonstrated advanced stages of the disease when compared with Group 2. Immunohistochemistry analysis suggests the presence of pre-existent vessels and neoformed vessels as well as predominant young cells in the injured parenchyma of Group 2. Immunohistochemistry analysis suggests that cell therapy would promote a reconstructive response. Histology and HRCT analysis suggest a positive application of swine as a model for a bleomicine inducing of fibrotic interstitial pulmonary disease. Microsc. Res. Tech. 74:957-962, 2011. (C) 2011 Wiley-Liss, Inc. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCP Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/InCor Artigos e Materiais de Revistas Científicas - LIM/09 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_CABRAL_Cell_Therapy_for_Fibrotic_Interstitial_Pulmonary_Disease_Experimental_2011.PDF Restricted Access | publishedVersion (English) | 547.4 kB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.