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https://observatorio.fm.usp.br/handle/OPI/23485
Title: | Differential elemental distribution of retained particles along the respiratory tract |
Authors: | SAIEG, Mauro A.; CURY, Patricia M.; GODLESKI, John J.; STEARNS, Rebecca; DUARTE, Luis G. P.; D'AGOSTINO, Liz; KAHN, Henrique; PINTO, Emilia M.; MAUAD, Thais; SALDIVA, Paulo H. N.; BERNARDI, Fabiola D. C. |
Citation: | INHALATION TOXICOLOGY, v.23, n.8, p.459-467, 2011 |
Abstract: | Objective: To compare the elemental profile of particles retained along the bronchial tree and lymph nodes by combining laser capture microdissection (LCM) and elemental composition analysis through energy dispersive x-ray (EDX) and scanning electron microscopy (SEM). Material and methods: Twenty-four right lung middle lobes from autopsied cases were obtained from two cities with different pollution backgrounds. Lung samples were collected from three distinct sites within the lung at the time of autopsy: peribronchial tissue, peripheral parenchyma and hilar lymph nodes. Areas of potentially increased particle deposition were microdissected using LCM and analyzed for elemental composition through EDX """"allied"""" with SEM. Results: Elemental analyses of the particles retained along the bronchial tree showed two groups of distribution: peribronchiolar or lymph node deposition. The elemental profile of peribronchial areas were significantly different between the two cities and were better discriminators of past air pollution exposure. Conclusion: Our data suggest that particle uptake varies along the bronchial tree and human lung tissue retains particles indicative of regional air pollution background. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - HC/InCor Artigos e Materiais de Revistas Científicas - LIM/05 |
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