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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | BOOPATHI, Ettickan | - |
dc.contributor.author | HYPOLITE, Joseph A. | - |
dc.contributor.author | ZDERIC, Stephen A. | - |
dc.contributor.author | GOMES, Cristiano Mendes | - |
dc.contributor.author | MALKOWICZ, Bruce | - |
dc.contributor.author | LIOU, Hsiou-Chi | - |
dc.contributor.author | WEIN, Alan J. | - |
dc.contributor.author | CHACKO, Samuel | - |
dc.date.accessioned | 2013-09-23T16:54:46Z | - |
dc.date.available | 2013-09-23T16:54:46Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | MOLECULAR AND CELLULAR BIOLOGY, v.33, n.5, p.1085-1102, 2013 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/2398 | - |
dc.description.abstract | Protein kinase C (PKC)-potentiated inhibitory protein of 17 kDa (CPI-17) inhibits myosin light chain phosphatase, altering the levels of myosin light chain phosphorylation and Ca2+ sensitivity in smooth muscle. In this study, we characterized the CPI-17 promoter and identified binding sites for GATA-6 and nuclear factor kappa B (NF-kappa B). GATA-6 and NF-kappa B upregulated CPI-17 expression in cultured human and mouse bladder smooth muscle (BSM) cells in an additive manner. CPI-17 expression was decreased upon GATA-6 silencing in cultured BSM cells and in BSM from NF-kappa B knockout (KO) mice. Moreover, force maintenance by BSM strips from KO mice was decreased compared with the force maintenance of BSM strips from wild-type mice. GATA-6 and NF-kappa B overexpression was associated with CPI-17 overexpression in BSM from men with benign prostatic hyperplasia (BPH)-induced bladder hypertrophy and in a mouse model of bladder outlet obstruction. Thus, aberrant expression of NF-kappa B and GATA-6 deregulates CPI-17 expression and the contractile function of smooth muscle. Our data provide insight into how GATA-6 and NF-kappa B mediate CPI-17 transcription, PKC-mediated signaling, and BSM remodeling associated with lower urinary tract symptoms in patients with BPH. | - |
dc.description.sponsorship | O'Brien Urology Research Center from NIH-NIDDK [P50 DK052620] | - |
dc.language.iso | eng | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.relation.ispartof | Molecular and Cellular Biology | - |
dc.rights | restrictedAccess | - |
dc.subject.other | bladder outlet obstruction | - |
dc.subject.other | protein-kinase-c | - |
dc.subject.other | nonmuscle myosin-ii | - |
dc.subject.other | urinary-bladder | - |
dc.subject.other | rho-kinase | - |
dc.subject.other | cardiac-hypertrophy | - |
dc.subject.other | up-regulation | - |
dc.subject.other | signal-transduction | - |
dc.subject.other | inhibitory protein | - |
dc.subject.other | binding-proteins | - |
dc.title | GATA-6 and NF-kappa B Activate CPI-17 Gene Transcription and Regulate Ca2+ Sensitization of Smooth Muscle Contraction | - |
dc.type | article | - |
dc.rights.holder | Copyright AMER SOC MICROBIOLOGY | - |
dc.identifier.doi | 10.1128/MCB.00626-12 | - |
dc.identifier.pmid | 23275439 | - |
dc.subject.wos | Biochemistry & Molecular Biology | - |
dc.subject.wos | Cell Biology | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | BOOPATHI, Ettickan:Univ Penn, Div Urol, Glenolden, PA 19036 USA | - |
hcfmusp.author.external | HYPOLITE, Joseph A.:Univ Penn, Div Urol, Glenolden, PA 19036 USA | - |
hcfmusp.author.external | ZDERIC, Stephen A.:Childrens Hosp Philadelphia, Dept Urol, Philadelphia, PA 19104 USA | - |
hcfmusp.author.external | MALKOWICZ, Bruce:Univ Penn, Div Urol, Glenolden, PA 19036 USA | - |
hcfmusp.author.external | LIOU, Hsiou-Chi:Cornell Univ, Weill Med Coll, Dept Med, Div Immunol, New York, NY 10021 USA | - |
hcfmusp.author.external | WEIN, Alan J.:Univ Penn, Div Urol, Glenolden, PA 19036 USA | - |
hcfmusp.author.external | CHACKO, Samuel:Univ Penn, Div Urol, Glenolden, PA 19036 USA; Univ Penn, Dept Pathol, Philadelphia, PA 19104 USA | - |
hcfmusp.description.beginpage | 1085 | - |
hcfmusp.description.endpage | 1102 | - |
hcfmusp.description.issue | 5 | - |
hcfmusp.description.volume | 33 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | 2-s2.0-84874704264 | - |
hcfmusp.origem.id | WOS:000317267800017 | - |
hcfmusp.publisher.city | WASHINGTON | - |
hcfmusp.publisher.country | USA | - |
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dc.description.index | MEDLINE | - |
hcfmusp.remissive.sponsorship | NIH | - |
hcfmusp.citation.scopus | 19 | - |
hcfmusp.scopus.lastupdate | 2024-04-12 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - HC/IOT Artigos e Materiais de Revistas Científicas - LIM/55 |
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